Detailed information of TA system TA052808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 3144436..3145857
• Replicon: chromosome
• Accession: NZ_CP010555
• Organism: Pseudomonas aeruginosa strain FRD1

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: EG09_RS14300
• Protein ID: WP_025297643.1
• Coordinates: 3144436..3145509 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: EG09_RS31560
• Protein ID: WP_158025315.1
• Coordinates: 3145564..3145857 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG09_RS14280	3140529..3140957	-	429	WP_025297641.1	S24 family peptidase	-
EG09_RS14285	3141237..3142820	+	1584	WP_025297642.1	hypothetical protein	-
EG09_RS14290	3142873..3143169	-	297	WP_023128612.1	hypothetical protein	-
EG09_RS14295	3143467..3143721	-	255	WP_023128610.1	hypothetical protein	-
EG09_RS14300	3144436..3145509	+	1074	WP_025297643.1	type II toxin-antitoxin system RnlA family toxin	Toxin
EG09_RS31560	3145564..3145857	+	294	WP_158025315.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
EG09_RS14305	3145996..3146789	-	794	Protein_2858	transporter substrate-binding domain-containing protein	-
EG09_RS31565	3146764..3146958	+	195	WP_023128607.1	OprD family outer membrane porin	-
EG09_RS14310	3147182..3147546	-	365	Protein_2860	DNA binding protein	-
EG09_RS14315	3147877..3148455	-	579	WP_025297644.1	hypothetical protein	-
EG09_RS14320	3148537..3148920	-	384	WP_025297645.1	hypothetical protein	-
EG09_RS14325	3148923..3149393	-	471	WP_023128604.1	hypothetical protein	-
EG09_RS14330	3149436..3149831	-	396	WP_023128603.1	hypothetical protein	-
EG09_RS14335	3149996..3150451	+	456	WP_025297646.1	helix-turn-helix domain-containing protein	-
EG09_RS14340	3150542..3150754	-	213	WP_023128602.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3110251..3145857	35606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal (6-87)

Antitoxin

• Antitoxin RnlB/LsoB (2-96)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40381.89 Da
• Isoelectric Point: 6.1830

>T52808 WP_025297643.1 NZ_CP010555:3144436-3145509 [Pseudomonas aeruginosa]
VTERDYKNINLKRCIIEETTHCFIRDNGLKLRSYGDKPGSSGLRVVFGKAGIEDATLDIFFTNVGASTLTYKVGKNQALG
QQLADALYETIHPDEFVTMNLGIKGVDLDDAEALIKELTESANADIEVASRTSADRKHIWKLKSKKNLDELTVTQHENTH
KLQIQGRPLSCYQQLSYLLTELLEINALECILFKKEENRSEFVCAEVAESVLKERFGDCYANLPQTSRRLLLASLCVRLA
SPALPDYCMLVYPELRTLEGAIKQKLAEKNLSNQEESFGGFFTKTNGQFYLKQQYHADVGDAKLQGHLDNAYTFFNKQRH
GLFHMEEMPSVSRMISNITQAVSLCDEAYDHIKNLYS

Nucleotide

• Length: 1074 bp

>T52808 NZ_CP010555:3144436-3145509 [Pseudomonas aeruginosa]
GTGACTGAACGCGACTACAAAAACATCAACCTCAAACGATGCATTATCGAGGAAACGACTCATTGCTTCATCCGAGATAA
TGGCCTGAAGCTGCGATCCTATGGTGACAAGCCTGGGAGTTCCGGTCTTCGAGTAGTCTTCGGAAAAGCAGGGATTGAAG
ATGCCACGCTTGATATTTTTTTCACGAATGTCGGTGCGTCTACCCTGACCTACAAGGTGGGCAAGAACCAAGCTCTCGGC
CAGCAGCTAGCGGATGCTCTTTACGAAACAATCCACCCCGATGAATTCGTAACGATGAACCTTGGTATCAAAGGCGTCGA
TCTGGATGATGCCGAAGCCCTTATCAAGGAACTCACTGAGTCAGCCAATGCGGACATTGAGGTTGCTTCGCGCACAAGCG
CTGATCGCAAGCATATTTGGAAGCTCAAGTCTAAAAAGAACTTGGATGAGTTGACTGTCACTCAGCACGAGAACACCCAT
AAACTGCAGATCCAGGGTCGTCCTCTCAGCTGCTATCAGCAGTTGAGCTACCTGCTCACCGAGCTCCTAGAGATCAACGC
ACTCGAATGCATCCTGTTCAAAAAGGAAGAAAATCGCTCGGAGTTCGTTTGTGCCGAGGTCGCGGAAAGCGTACTCAAGG
AAAGGTTTGGCGACTGCTACGCCAACCTGCCACAGACCTCGCGTAGGCTGCTCCTGGCAAGCCTTTGCGTGAGGCTTGCC
AGCCCCGCGCTACCCGACTACTGCATGCTGGTTTATCCGGAACTCCGCACCCTTGAAGGCGCGATCAAACAGAAGCTTGC
AGAAAAAAACCTTTCAAATCAGGAAGAAAGCTTCGGTGGCTTCTTTACCAAGACCAATGGTCAGTTTTATCTGAAGCAGC
AATACCATGCTGATGTCGGCGATGCTAAACTACAGGGTCATCTGGACAACGCTTACACCTTCTTCAACAAGCAACGCCAC
GGATTGTTCCACATGGAAGAGATGCCATCCGTTAGTCGAATGATTAGCAACATCACCCAAGCTGTGTCATTGTGTGATGA
AGCATATGATCATATCAAGAATTTGTATAGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11039.38 Da
• Isoelectric Point: 4.5163

>AT52808 WP_158025315.1 NZ_CP010555:3145564-3145857 [Pseudomonas aeruginosa]
MTVVTATSYENPLSRLSIIASEMRNTSHSSKEIVLFDLLCSNGEEWNRFVSINYNGTDFEKSTCSIVSKSDIPTDLLETQ
TRFFQIHPQYLLDSVLN

Nucleotide

• Length: 294 bp

>AT52808 NZ_CP010555:3145564-3145857 [Pseudomonas aeruginosa]
ATGACGGTAGTTACCGCTACCAGTTATGAGAACCCCTTATCTCGTCTTTCTATTATTGCTAGCGAGATGCGCAATACATC
TCACTCCAGCAAGGAGATAGTTCTATTCGATCTACTCTGCTCTAACGGAGAAGAGTGGAATCGTTTCGTCTCCATCAATT
ACAACGGCACCGACTTCGAAAAAAGCACCTGTAGCATTGTCTCCAAGTCCGACATCCCTACAGACCTCTTGGAAACACAA
ACTCGCTTCTTTCAAATCCATCCACAATATCTGCTTGATTCAGTTTTGAATTAA