Detailed information of TA system TA052805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1429981..1431023
• Replicon: chromosome
• Accession: NZ_CP010555
• Organism: Pseudomonas aeruginosa strain FRD1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EG09_RS06245
• Protein ID: WP_003153636.1
• Coordinates: 1429981..1430556 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: EG09_RS06250
• Protein ID: WP_003050245.1
• Coordinates: 1430553..1431023 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG09_RS06220	1426419..1427144	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
EG09_RS06225	1427183..1428085	-	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
EG09_RS06230	1428085..1428585	-	501	WP_003090159.1	hypothetical protein	-
EG09_RS06235	1428582..1429052	-	471	WP_003090160.1	hypothetical protein	-
EG09_RS06240	1429049..1429963	-	915	WP_016852809.1	AAA family ATPase	-
EG09_RS06245	1429981..1430556	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
EG09_RS06250	1430553..1431023	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
EG09_RS31225	1431227..1431595	+	369	Protein_1253	RAQPRD family integrative conjugative element protein	-
EG09_RS06260	1431606..1431839	+	234	WP_023101201.1	TIGR03758 family integrating conjugative element protein	-
EG09_RS06265	1431856..1432215	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
EG09_RS06270	1432227..1432625	+	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
EG09_RS06275	1432622..1433314	+	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
EG09_RS06280	1433311..1434222	+	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
EG09_RS06285	1434212..1435630	+	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T52805 WP_003153636.1 NZ_CP010555:c1430556-1429981 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T52805 NZ_CP010555:c1430556-1429981 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT52805 WP_003050245.1 NZ_CP010555:c1431023-1430553 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT52805 NZ_CP010555:c1431023-1430553 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68