Detailed information of TA system TA052804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 473530..474751
• Replicon: chromosome
• Accession: NZ_CP010555
• Organism: Pseudomonas aeruginosa strain FRD1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: EG09_RS02240
• Protein ID: WP_003110488.1
• Coordinates: 473530..474108 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EG09_RS02245
• Protein ID: WP_003088112.1
• Coordinates: 474197..474751 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG09_RS02225	468806..470083	+	1278	WP_003102743.1	TolC family protein	-
EG09_RS02230	470073..472244	+	2172	WP_025297367.1	type I secretion system permease/ATPase	-
EG09_RS02235	472234..473421	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
EG09_RS02240	473530..474108	-	579	WP_003110488.1	HD domain-containing protein	Toxin
EG09_RS02245	474197..474751	-	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
EG09_RS02250	474909..477104	-	2196	WP_003122807.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
EG09_RS02255	477109..477570	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
EG09_RS02260	477901..478224	+	324	WP_015648646.1	multidrug efflux SMR transporter	-
EG09_RS02265	478237..478635	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
EG09_RS02270	478632..479183	-	552	WP_003450159.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T52804 WP_003110488.1 NZ_CP010555:c474108-473530 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T52804 NZ_CP010555:c474108-473530 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTTTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTTCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAAGCGGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCATCTGGACAC
GCCGCTGCGCGATCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGACCGCCGAAGCCCTGGCAGTGAAAGCGC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT52804 WP_003088112.1 NZ_CP010555:c474751-474197 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT52804 NZ_CP010555:c474751-474197 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCATCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4