Detailed information of TA system TA052800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-AbrB
• Location: 1842894..1843458
• Replicon: chromosome
• Accession: NZ_CP010554
• Organism: Rugosibacter aromaticivorans strain Ca6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: PG1C_RS09250
• Protein ID: WP_202634517.1
• Coordinates: 1842894..1843241 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PG1C_RS09255
• Protein ID: WP_202634518.1
• Coordinates: 1843231..1843458 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PG1C_RS09205	1837922..1838281	-	360	WP_202634508.1	hypothetical protein	-
PG1C_RS09210	1838284..1838907	-	624	WP_202634509.1	HTH domain-containing protein	-
PG1C_RS09215	1839067..1839483	-	417	WP_202634510.1	HI0074 family nucleotidyltransferase substrate-binding subunit	-
PG1C_RS09220	1839474..1839770	-	297	WP_202634511.1	nucleotidyltransferase domain-containing protein	-
PG1C_RS09225	1839739..1840248	-	510	WP_202634512.1	adenylyl-sulfate kinase	-
PG1C_RS09230	1840245..1840778	-	534	WP_202634513.1	hypothetical protein	-
PG1C_RS09235	1840775..1841074	-	300	WP_202634514.1	nucleotidyltransferase domain-containing protein	-
PG1C_RS09240	1841435..1842313	-	879	WP_202634515.1	ABC transporter permease	-
PG1C_RS09245	1842310..1842855	-	546	WP_202634516.1	dTDP-4-dehydrorhamnose 3,5-epimerase	-
PG1C_RS09250	1842894..1843241	-	348	WP_202634517.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PG1C_RS09255	1843231..1843458	-	228	WP_202634518.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PG1C_RS09260	1843529..1844443	-	915	WP_202634519.1	glucose-1-phosphate thymidylyltransferase RfbA	-
PG1C_RS09265	1844440..1844604	-	165	WP_202637004.1	hypothetical protein	-
PG1C_RS09270	1844635..1845543	-	909	WP_202634520.1	dTDP-4-dehydrorhamnose reductase	-
PG1C_RS09275	1845540..1846667	-	1128	WP_202634521.1	dTDP-glucose 4,6-dehydratase	-
PG1C_RS09280	1846894..1847535	-	642	WP_202634522.1	phosphatase PAP2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

• SpoVT-AbrB domain (5-46)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12977.05 Da
• Isoelectric Point: 7.4229

>T52800 WP_202634517.1 NZ_CP010554:c1843241-1842894 [Rugosibacter aromaticivorans]
VTFNAFDVVAVPFPFTDREASKRRPALVISNDRFNLNHDHMVLAMITTATKTVWPSDVVINHWQEAGLKVPCRVRLKLFT
LDNHLILKTVGHLLPQDVQSVQAVLTEYLDVASTI

Nucleotide

• Length: 348 bp

>T52800 NZ_CP010554:c1843241-1842894 [Rugosibacter aromaticivorans]
GTGACCTTTAATGCATTTGATGTCGTTGCCGTTCCTTTTCCATTCACTGATCGTGAAGCATCCAAGCGACGCCCGGCCTT
GGTAATATCGAATGACCGTTTTAATCTAAATCACGATCATATGGTATTAGCCATGATCACCACTGCCACTAAAACGGTTT
GGCCTAGTGATGTTGTAATAAACCACTGGCAAGAAGCCGGACTTAAAGTGCCATGCCGTGTTCGGTTAAAACTGTTTACT
TTAGATAACCACCTGATTTTGAAAACTGTAGGACATTTATTACCTCAAGATGTACAGTCAGTGCAGGCCGTCTTAACTGA
ATACCTTGATGTTGCCAGCACCATTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8628.61 Da
• Isoelectric Point: 4.4929

>AT52800 WP_202634518.1 NZ_CP010554:c1843458-1843231 [Rugosibacter aromaticivorans]
MEFARVTAKGQATIPRRIREAAHIREGDMLAFELDSNNRITIKRIESSVDTELAALQETLSEWNSPQDEEAWRDL

Nucleotide

• Length: 228 bp

>AT52800 NZ_CP010554:c1843458-1843231 [Rugosibacter aromaticivorans]
ATGGAATTTGCCCGCGTGACAGCTAAAGGCCAGGCAACCATCCCCAGAAGAATCAGAGAAGCGGCACACATTAGGGAAGG
CGATATGCTGGCTTTTGAATTAGATAGCAACAACCGGATCACCATCAAGCGTATTGAGTCAAGCGTTGATACCGAGCTGG
CTGCACTGCAAGAAACACTGTCGGAATGGAATTCCCCTCAGGATGAGGAAGCCTGGCGTGACCTTTAA