Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2787856..2788436 Replicon chromosome
Accession NZ_CP010537
Organism Cupriavidus basilensis strain 4G11

Toxin (Protein)


Gene name vapC Uniprot ID A0A0C4YEG8
Locus tag RR42_RS32930 Protein ID WP_043356151.1
Coordinates 2787856..2788224 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0C4YM66
Locus tag RR42_RS32935 Protein ID WP_043358332.1
Coordinates 2788218..2788436 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR42_RS32910 2783454..2784368 + 915 WP_043356148.1 LysR family transcriptional regulator -
RR42_RS32915 2784405..2785283 - 879 WP_043356149.1 NAD(P)-dependent oxidoreductase -
RR42_RS32920 2785336..2786403 - 1068 WP_043356150.1 maleylacetate reductase -
RR42_RS32925 2786743..2787735 + 993 WP_052495131.1 alpha/beta fold hydrolase -
RR42_RS32930 2787856..2788224 - 369 WP_043356151.1 type II toxin-antitoxin system VapC family toxin Toxin
RR42_RS32935 2788218..2788436 - 219 WP_043358332.1 ribbon-helix-helix protein, CopG family Antitoxin
RR42_RS32940 2788716..2790446 + 1731 WP_043356153.1 nitrite/sulfite reductase -
RR42_RS32945 2790439..2791023 + 585 WP_043356154.1 DUF934 domain-containing protein -
RR42_RS32950 2790989..2791843 - 855 WP_043356156.1 alpha/beta hydrolase -
RR42_RS32955 2791972..2793354 - 1383 WP_043356158.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-41)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13840.06 Da        Isoelectric Point: 6.4728

>T52793 WP_043356151.1 NZ_CP010537:c2788224-2787856 [Cupriavidus basilensis]
VVNALFDTNILIDHLNGIEEAMIEMRRYESRAISVITWMEVMVGATVEEEQTLRAWLNAFRLIAVDDAVSTRAVQIRKTA
RIKLPDAIIWASAQAHSMLLVTRNTKDFSVSEPGVRIPYQLK

Download         Length: 369 bp

>T52793 NZ_CP010537:c2788224-2787856 [Cupriavidus basilensis]
GTGGTGAACGCGCTCTTCGACACCAATATCCTGATCGACCACTTGAACGGAATCGAAGAGGCCATGATCGAGATGCGCCG
CTACGAGTCAAGGGCGATCAGTGTCATCACCTGGATGGAGGTGATGGTGGGCGCAACAGTGGAAGAAGAGCAGACATTGC
GCGCGTGGCTCAATGCGTTCCGGCTGATCGCAGTCGATGATGCGGTTTCCACTCGCGCAGTCCAGATTCGAAAAACCGCA
CGAATCAAGCTGCCTGACGCGATTATCTGGGCGTCGGCGCAAGCGCATTCGATGTTGCTGGTCACACGCAATACCAAAGA
CTTCTCGGTCAGCGAGCCTGGTGTGCGGATTCCCTATCAGCTCAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8173.43 Da        Isoelectric Point: 7.0115

>AT52793 WP_043358332.1 NZ_CP010537:c2788436-2788218 [Cupriavidus basilensis]
MSRILVDLAEDQLQALAAIVEAEKRPRAVVIREAIQAYIAQRKPGLAADTFGLWKHRGVDGLAYQEKLREEW

Download         Length: 219 bp

>AT52793 NZ_CP010537:c2788436-2788218 [Cupriavidus basilensis]
ATGAGTCGCATCCTGGTCGACCTTGCGGAAGACCAGCTCCAGGCGCTGGCAGCCATTGTCGAGGCAGAAAAGCGCCCTCG
GGCAGTAGTCATTCGCGAGGCAATTCAAGCGTACATCGCGCAGCGCAAGCCTGGCCTGGCCGCTGACACCTTCGGGCTTT
GGAAGCACCGCGGGGTGGATGGCCTTGCGTATCAGGAAAAGCTGCGGGAGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C4YEG8

Antitoxin

Source ID Structure
AlphaFold DB A0A0C4YM66

References