Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 5468..6069 Replicon plasmid LBPp7
Accession NZ_CP010527
Organism Lactobacillus plantarum subsp. plantarum P-8

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag LBP_RS15390 Protein ID WP_001748110.1
Coordinates 5468..5812 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag LBP_RS15395 Protein ID WP_001748109.1
Coordinates 5806..6069 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBP_RS15360 1417..2079 + 663 WP_003645001.1 amino acid ABC transporter permease -
LBP_RS15365 2060..2749 + 690 WP_003645002.1 amino acid ABC transporter permease -
LBP_RS15370 2742..3482 + 741 WP_015639667.1 amino acid ABC transporter ATP-binding protein -
LBP_RS15375 3503..4357 + 855 WP_021816930.1 transporter substrate-binding domain-containing protein -
LBP_RS15390 5468..5812 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LBP_RS15395 5806..6069 - 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LBP_RS15400 6155..6742 + 588 WP_003646144.1 site-specific integrase -
LBP_RS15405 9275..9835 - 561 WP_003643342.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..15174 15174


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T52787 WP_001748110.1 NZ_CP010527:c5812-5468 [Lactobacillus plantarum subsp. plantarum P-8]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T52787 NZ_CP010527:c5812-5468 [Lactobacillus plantarum subsp. plantarum P-8]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCGAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGCAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT52787 WP_001748109.1 NZ_CP010527:c6069-5806 [Lactobacillus plantarum subsp. plantarum P-8]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT52787 NZ_CP010527:c6069-5806 [Lactobacillus plantarum subsp. plantarum P-8]
ATGGAACTAATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References