Detailed information of TA system TA052779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2181616..2182145
• Replicon: chromosome
• Accession: NZ_CP010526
• Organism: Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RU53_RS11015
• Protein ID: WP_000621175.1
• Coordinates: 2181616..2181978 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RU53_RS11020
• Protein ID: WP_000948331.1
• Coordinates: 2181975..2182145 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RU53_RS10990	2178595..2179365	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
RU53_RS10995	2179340..2179819	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
RU53_RS11000	2179821..2180147	-	327	WP_001052491.1	anti-sigma factor antagonist	-
RU53_RS11005	2180266..2181267	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
RU53_RS11015	2181616..2181978	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RU53_RS11020	2181975..2182145	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RU53_RS11025	2182230..2183378	-	1149	WP_001281154.1	alanine racemase	-
RU53_RS11030	2183444..2183803	-	360	WP_000581197.1	holo-ACP synthase	-
RU53_RS11035	2183807..2184298	-	492	WP_001205912.1	PH domain-containing protein	-
RU53_RS11040	2184285..2185868	-	1584	WP_001294620.1	PH domain-containing protein	-
RU53_RS11045	2185861..2186340	-	480	WP_001287079.1	hypothetical protein	-
RU53_RS11050	2186549..2187109	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T52779 WP_000621175.1 NZ_CP010526:c2181978-2181616 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T52779 NZ_CP010526:c2181978-2181616 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT52779 WP_000948331.1 NZ_CP010526:c2182145-2181975 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT52779 NZ_CP010526:c2182145-2181975 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1