Detailed information of TA system TA052778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2019624..2020400
• Replicon: chromosome
• Accession: NZ_CP010526
• Organism: Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: RU53_RS15530
• Protein ID: WP_000031108.1
• Coordinates: 2019624..2019776 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: RU53_RS10075
• Protein ID: WP_001251228.1
• Coordinates: 2019801..2020400 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RU53_RS10050	2015310..2016629	-	1320	WP_001837615.1	ISL3 family transposase	-
RU53_RS10055	2016756..2018141	-	1386	WP_000116229.1	class II fumarate hydratase	-
RU53_RS10060	2018337..2018732	-	396	WP_000901023.1	hypothetical protein	-
RU53_RS15530	2019624..2019776	-	153	WP_000031108.1	hypothetical protein	Toxin
RU53_RS10075	2019801..2020400	-	600	WP_001251228.1	glucosamine-6-phosphate isomerase	Antitoxin
RU53_RS10080	2020558..2021028	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
RU53_RS10085	2021033..2022160	-	1128	WP_000379985.1	tRNA epoxyqueuosine(34) reductase QueG	-
RU53_RS10090	2022311..2023033	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
RU53_RS10095	2023026..2024483	-	1458	WP_000649907.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2015310..2016629	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T52778 WP_000031108.1 NZ_CP010526:c2019776-2019624 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T52778 NZ_CP010526:c2019776-2019624 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT52778 WP_001251228.1 NZ_CP010526:c2020400-2019801 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEGLGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNIILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT52778 NZ_CP010526:c2020400-2019801 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGGGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATATTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6