Detailed information of TA system TA052766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 888548..889077
• Replicon: chromosome
• Accession: NZ_CP010526
• Organism: Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: RU53_RS04405
• Protein ID: WP_001103939.1
• Coordinates: 888760..889077 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IA75
• Locus tag: RU53_RS04400
• Protein ID: WP_001058492.1
• Coordinates: 888548..888757 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RU53_RS04370	884878..885342	+	465	WP_001085185.1	SsrA-binding protein SmpB	-
RU53_RS04375	885905..887011	-	1107	WP_000149511.1	site-specific integrase	-
RU53_RS04380	887001..887804	-	804	WP_000358990.1	helix-turn-helix domain-containing protein	-
RU53_RS04385	887940..888143	+	204	WP_001045296.1	transcriptional regulator	-
RU53_RS04390	888179..888397	+	219	WP_000163544.1	helix-turn-helix domain-containing protein	-
RU53_RS04395	888409..888555	+	147	WP_000784885.1	hypothetical protein	-
RU53_RS04400	888548..888757	+	210	WP_001058492.1	hypothetical protein	Antitoxin
RU53_RS04405	888760..889077	+	318	WP_001103939.1	DUF1474 family protein	Toxin
RU53_RS04410	889141..890010	+	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
RU53_RS04415	890027..891484	+	1458	WP_000390453.1	virulence-associated E family protein	-
RU53_RS04420	891785..892147	+	363	WP_001039170.1	hypothetical protein	-
RU53_RS04425	892149..892433	+	285	WP_000998185.1	hypothetical protein	-
RU53_RS04430	892430..893071	+	642	WP_001019807.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	dfrG	sec / sell	882484..908411	25927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T52766 WP_001103939.1 NZ_CP010526:888760-889077 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T52766 NZ_CP010526:888760-889077 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCACAAAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8238.28 Da
• Isoelectric Point: 4.3718

>AT52766 WP_001058492.1 NZ_CP010526:888548-888757 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT52766 NZ_CP010526:888548-888757 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IA75