Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 258629..259262 Replicon chromosome
Accession NZ_CP010525
Organism Weizmannia coagulans strain HM-08

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag SB48_RS01225 Protein ID WP_013860680.1
Coordinates 258912..259262 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag SB48_RS01220 Protein ID WP_014096808.1
Coordinates 258629..258907 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SB48_RS01200 254736..255335 - 600 WP_014096804.1 rhomboid family intramembrane serine protease -
SB48_RS01205 255616..255966 + 351 WP_014096805.1 holo-ACP synthase -
SB48_RS01210 256107..257123 + 1017 WP_035183268.1 outer membrane lipoprotein carrier protein LolA -
SB48_RS01215 257325..258476 + 1152 WP_014096807.1 alanine racemase -
SB48_RS01220 258629..258907 + 279 WP_014096808.1 hypothetical protein Antitoxin
SB48_RS01225 258912..259262 + 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SB48_RS01235 259752..260579 + 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
SB48_RS01240 260583..260939 + 357 WP_014096810.1 STAS domain-containing protein -
SB48_RS01245 260944..261345 + 402 WP_014096811.1 anti-sigma regulatory factor -
SB48_RS01250 261359..262369 + 1011 WP_017551130.1 PP2C family protein-serine/threonine phosphatase -
SB48_RS01255 262439..262774 + 336 WP_026104587.1 anti-sigma factor antagonist -
SB48_RS01260 262778..263248 + 471 WP_014096814.1 anti-sigma B factor RsbW -
SB48_RS01265 263226..264005 + 780 WP_014096815.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T52765 WP_013860680.1 NZ_CP010525:258912-259262 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T52765 NZ_CP010525:258912-259262 [Weizmannia coagulans]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGTTTTAGCCCCACTGTCATTGTTGCCGCGATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATTGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAAAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT52765 WP_014096808.1 NZ_CP010525:258629-258907 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT52765 NZ_CP010525:258629-258907 [Weizmannia coagulans]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAATTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACGATGAGGCGTGGTTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References