Detailed information of TA system TA052743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 995780..996384
• Replicon: chromosome
• Accession: NZ_CP010517
• Organism: Cupriavidus gilardii CR3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0M4L7D5
• Locus tag: CR3_RS20035
• Protein ID: WP_053823864.1
• Coordinates: 996079..996384 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CR3_RS25240
• Protein ID: WP_082371731.1
• Coordinates: 995780..996082 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR3_RS20010	991050..992027	-	978	WP_053823860.1	tripartite tricarboxylate transporter substrate binding protein	-
CR3_RS20015	992257..993000	+	744	WP_053823861.1	GntR family transcriptional regulator	-
CR3_RS20020	993060..993431	+	372	WP_053823862.1	cupin domain-containing protein	-
CR3_RS20025	993554..994312	+	759	WP_053823863.1	glucose 1-dehydrogenase	-
CR3_RS20030	994421..995701	+	1281	Protein_867	MFS transporter	-
CR3_RS25240	995780..996082	-	303	WP_082371731.1	putative addiction module antidote protein	Antitoxin
CR3_RS20035	996079..996384	-	306	WP_053823864.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR3_RS20040	996629..997822	-	1194	WP_053823865.1	MFS transporter	-
CR3_RS20045	998096..998491	+	396	WP_053823866.1	DUF1311 domain-containing protein	-
CR3_RS20050	998507..998746	-	240	WP_053823867.1	hypothetical protein	-
CR3_RS20055	998743..1000917	-	2175	WP_053823868.1	S8 family serine peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-85)

Antitoxin

• Uncharacterized protein HI1420 (7-94)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11236.97 Da
• Isoelectric Point: 10.7003

>T52743 WP_053823864.1 NZ_CP010517:c996384-996079 [Cupriavidus gilardii CR3]
MIEIREYPPFRRWLSGLPDEATKAVIARRLMRLSVGNFGDAKSVGGGVSELRIDDGPGFRVYFAREGPRVVLLLGGGDKS
TQHLDIRKAQALWAEIKRTQA

Nucleotide

• Length: 306 bp

>T52743 NZ_CP010517:c996384-996079 [Cupriavidus gilardii CR3]
ATGATAGAAATTCGCGAGTACCCGCCGTTCCGAAGGTGGCTGAGCGGACTGCCCGACGAGGCCACCAAGGCTGTGATCGC
TCGACGGCTGATGCGGCTGTCCGTCGGCAACTTCGGCGACGCCAAATCCGTTGGAGGTGGAGTTTCAGAGCTTCGCATCG
ACGACGGACCGGGTTTCCGCGTTTACTTCGCCCGCGAAGGCCCCCGCGTGGTGCTGCTGCTTGGTGGTGGGGACAAGTCG
ACGCAACACCTCGACATACGCAAGGCGCAAGCGCTGTGGGCAGAAATCAAGAGGACGCAAGCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10773.23 Da
• Isoelectric Point: 4.6290

>AT52743 WP_082371731.1 NZ_CP010517:c996082-995780 [Cupriavidus gilardii CR3]
MKDLEYRTFDPARYLDTDEARAAFLADALMDADHPELFAEALAAVARSRGNIAETAQAAGLSTSGLYKILGADSDPKLST
LRALLDVLGVELTIRPKLAH

Nucleotide

• Length: 303 bp

>AT52743 NZ_CP010517:c996082-995780 [Cupriavidus gilardii CR3]
ATGAAGGATCTGGAATATCGTACGTTCGACCCAGCGCGGTATCTCGACACCGACGAGGCGCGCGCCGCGTTTCTCGCGGA
CGCGCTGATGGATGCGGATCATCCTGAGTTGTTCGCCGAAGCACTGGCCGCAGTCGCACGCTCGCGCGGCAACATTGCCG
AGACGGCGCAGGCCGCCGGGTTGTCAACCTCGGGGCTCTACAAGATTCTGGGCGCCGACAGCGATCCGAAACTCTCGACG
TTGCGGGCCTTGCTCGATGTACTGGGCGTTGAACTGACGATCCGTCCTAAGCTCGCGCACTAA

Structures

Toxin

• AlphaFold DB: A0A0M4L7D5