Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1180268..1180901 Replicon chromosome
Accession NZ_CP010516
Organism Cupriavidus gilardii CR3

Toxin (Protein)


Gene name hicA Uniprot ID A0A0M5KT30
Locus tag CR3_RS05300 Protein ID WP_053821538.1
Coordinates 1180725..1180901 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0M4KW28
Locus tag CR3_RS05295 Protein ID WP_053821537.1
Coordinates 1180268..1180684 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR3_RS25540 1176563..1176697 + 135 Protein_1059 DUF2514 family protein -
CR3_RS05275 1176673..1177296 - 624 WP_158507252.1 superinfection exclusion B family protein -
CR3_RS05280 1177331..1177693 - 363 WP_053821535.1 hypothetical protein -
CR3_RS25545 1178190..1178576 - 387 WP_144425761.1 hypothetical protein -
CR3_RS05285 1178890..1179354 - 465 WP_158507253.1 ImmA/IrrE family metallo-endopeptidase -
CR3_RS05290 1179365..1179760 - 396 WP_053823050.1 helix-turn-helix domain-containing protein -
CR3_RS05295 1180268..1180684 - 417 WP_053821537.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CR3_RS05300 1180725..1180901 - 177 WP_053821538.1 type II toxin-antitoxin system HicA family toxin Toxin
CR3_RS05305 1181065..1181883 - 819 WP_053821539.1 inositol monophosphatase -
CR3_RS05310 1182079..1183011 + 933 WP_053821540.1 RNA methyltransferase -
CR3_RS05315 1183372..1184115 + 744 WP_006577564.1 serine O-acetyltransferase -
CR3_RS05320 1184166..1184948 - 783 WP_053821541.1 UDP-2,3-diacylglucosamine diphosphatase -
CR3_RS05325 1185065..1185565 - 501 WP_023264649.1 peptidyl-prolyl cis-trans isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(82-130)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6588.65 Da        Isoelectric Point: 11.9145

>T52740 WP_053821538.1 NZ_CP010516:c1180901-1180725 [Cupriavidus gilardii CR3]
VKQSEFRRWLGRQGATFADGGKHLKVYLNGRQSTMPRHPGKEIAERTRKAILKQLGLA

Download         Length: 177 bp

>T52740 NZ_CP010516:c1180901-1180725 [Cupriavidus gilardii CR3]
GTGAAGCAGAGCGAGTTCAGACGCTGGCTAGGCCGGCAAGGCGCGACCTTCGCCGACGGCGGAAAGCACCTGAAGGTCTA
TCTCAACGGCCGACAGAGCACGATGCCGCGGCATCCCGGAAAGGAAATCGCCGAACGCACGCGCAAGGCCATCCTGAAGC
AACTTGGATTGGCGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15365.64 Da        Isoelectric Point: 4.4634

>AT52740 WP_053821537.1 NZ_CP010516:c1180684-1180268 [Cupriavidus gilardii CR3]
MLRYPVILTADDDGVAVSFPDIPEALTCGDSHEDALEMAADALVTAMEFYFEDRRPVPMPSAPKVGQELVDLPASLSAKV
LLLNEMLSQKVSQSELARRLDTRPQEVQRIVNLDHTTKIDTIEAAFRALGRRMELRIT

Download         Length: 417 bp

>AT52740 NZ_CP010516:c1180684-1180268 [Cupriavidus gilardii CR3]
ATGCTGCGCTACCCCGTCATCCTCACCGCCGACGACGATGGTGTGGCGGTCTCCTTCCCGGACATCCCCGAGGCCTTGAC
TTGCGGCGACTCGCACGAGGACGCCTTGGAGATGGCCGCCGATGCGCTTGTGACGGCGATGGAGTTCTACTTCGAGGATC
GGCGGCCTGTGCCGATGCCCTCGGCTCCCAAGGTGGGGCAGGAACTGGTCGACTTGCCCGCGAGCCTGTCCGCGAAGGTC
TTGCTGCTGAACGAAATGCTTTCGCAGAAGGTCAGCCAGTCGGAACTGGCACGGCGCCTGGATACCCGACCGCAGGAAGT
CCAGCGCATCGTCAATCTCGATCACACGACCAAGATCGACACGATCGAGGCTGCCTTTCGAGCGCTCGGCAGGCGGATGG
AACTGCGAATTACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5KT30

Antitoxin

Source ID Structure
AlphaFold DB A0A0M4KW28

References