Detailed information of TA system TA052723

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2873903..2874734
• Replicon: chromosome
• Accession: NZ_CP010445
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: SF31_RS14390
• Protein ID: WP_000854814.1
• Coordinates: 2874360..2874734 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: SF31_RS14385
• Protein ID: WP_001285584.1
• Coordinates: 2873903..2874271 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF31_RS14370	2871570..2873102	+	1533	WP_001350525.1	hypothetical protein	-
SF31_RS25545	2873099..2873545	+	447	WP_000187523.1	RadC family protein	-
SF31_RS14380	2873608..2873829	+	222	WP_000692323.1	DUF987 domain-containing protein	-
SF31_RS14385	2873903..2874271	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
SF31_RS14390	2874360..2874734	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
SF31_RS14395	2874731..2874925	+	195	WP_000988600.1	hypothetical protein	-
SF31_RS26250	2874938..2875051	+	114	WP_001161660.1	DUF957 domain-containing protein	-
SF31_RS24605	2875340..2875468	-	129	Protein_2715	transposase domain-containing protein	-
SF31_RS14405	2875540..2875722	+	183	WP_001016348.1	hypothetical protein	-
SF31_RS14410	2875823..2876152	-	330	WP_000450409.1	DUF496 family protein	-
SF31_RS14415	2876324..2877382	-	1059	WP_001200891.1	FUSC family protein	-
SF31_RS14420	2877580..2878053	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
SF31_RS14425	2878172..2879338	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T52723 WP_000854814.1 NZ_CP010445:2874360-2874734 [Escherichia coli K-12]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T52723 NZ_CP010445:2874360-2874734 [Escherichia coli K-12]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT52723 WP_001285584.1 NZ_CP010445:2873903-2874271 [Escherichia coli K-12]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT52723 NZ_CP010445:2873903-2874271 [Escherichia coli K-12]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6