Detailed information of TA system TA052712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2305654..2306292
• Replicon: chromosome
• Accession: NZ_CP010445
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: SF31_RS11460
• Protein ID: WP_000813794.1
• Coordinates: 2305654..2305830 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: SF31_RS11465
• Protein ID: WP_001270286.1
• Coordinates: 2305876..2306292 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF31_RS11440	2301273..2302487	-	1215	WP_001326689.1	BenE family transporter YdcO	-
SF31_RS11445	2302540..2303076	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
SF31_RS11450	2303149..2305110	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
SF31_RS11455	2305202..2305432	-	231	WP_000494244.1	YncJ family protein	-
SF31_RS11460	2305654..2305830	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
SF31_RS11465	2305876..2306292	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
SF31_RS11470	2306371..2307777	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
SF31_RS11475	2308022..2309167	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
SF31_RS11480	2309185..2310198	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
SF31_RS11485	2310199..2311140	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T52712 WP_000813794.1 NZ_CP010445:2305654-2305830 [Escherichia coli K-12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T52712 NZ_CP010445:2305654-2305830 [Escherichia coli K-12]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT52712 WP_001270286.1 NZ_CP010445:2305876-2306292 [Escherichia coli K-12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT52712 NZ_CP010445:2305876-2306292 [Escherichia coli K-12]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB