Detailed information of TA system TA052703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1293217..1293835
• Replicon: chromosome
• Accession: NZ_CP010445
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: SF31_RS06410
• Protein ID: WP_001290581.1
• Coordinates: 1293217..1293435 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: SF31_RS06415
• Protein ID: WP_000344800.1
• Coordinates: 1293461..1293835 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF31_RS06380	1288506..1289078	+	573	WP_000779842.1	YbaY family lipoprotein	-
SF31_RS06385	1289109..1289420	-	312	WP_000409911.1	MGMT family protein	-
SF31_RS06390	1289799..1290152	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
SF31_RS06395	1290194..1291744	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
SF31_RS06400	1291908..1292378	-	471	WP_000136192.1	YlaC family protein	-
SF31_RS06405	1292494..1293045	-	552	WP_000102564.1	maltose O-acetyltransferase	-
SF31_RS06410	1293217..1293435	-	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
SF31_RS06415	1293461..1293835	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
SF31_RS06420	1294381..1297530	-	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
SF31_RS06425	1297553..1298746	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T52703 WP_001290581.1 NZ_CP010445:c1293435-1293217 [Escherichia coli K-12]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T52703 NZ_CP010445:c1293435-1293217 [Escherichia coli K-12]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT52703 WP_000344800.1 NZ_CP010445:c1293835-1293461 [Escherichia coli K-12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT52703 NZ_CP010445:c1293835-1293461 [Escherichia coli K-12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5