Detailed information of TA system TA052681

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2821901..2822732
• Replicon: chromosome
• Accession: NZ_CP010444
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: SH07_RS14070
• Protein ID: WP_000854814.1
• Coordinates: 2822358..2822732 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: SH07_RS14065
• Protein ID: WP_001285584.1
• Coordinates: 2821901..2822269 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH07_RS14050	2819568..2821100	+	1533	WP_001350525.1	hypothetical protein	-
SH07_RS25125	2821097..2821543	+	447	WP_000187523.1	RadC family protein	-
SH07_RS14060	2821606..2821827	+	222	WP_000692323.1	DUF987 domain-containing protein	-
SH07_RS14065	2821901..2822269	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
SH07_RS14070	2822358..2822732	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
SH07_RS14075	2822729..2822923	+	195	WP_000988600.1	hypothetical protein	-
SH07_RS25825	2822936..2823049	+	114	WP_001161660.1	DUF957 domain-containing protein	-
SH07_RS24185	2823338..2823466	-	129	Protein_2656	transposase domain-containing protein	-
SH07_RS14085	2823538..2823720	+	183	WP_001016348.1	hypothetical protein	-
SH07_RS14090	2823821..2824150	-	330	WP_000450409.1	DUF496 family protein	-
SH07_RS14095	2824322..2825380	-	1059	WP_001200891.1	FUSC family protein	-
SH07_RS14100	2825578..2826051	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
SH07_RS14105	2826170..2827336	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T52681 WP_000854814.1 NZ_CP010444:2822358-2822732 [Escherichia coli K-12]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T52681 NZ_CP010444:2822358-2822732 [Escherichia coli K-12]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT52681 WP_001285584.1 NZ_CP010444:2821901-2822269 [Escherichia coli K-12]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT52681 NZ_CP010444:2821901-2822269 [Escherichia coli K-12]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6