Detailed information of TA system TA052661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1242415..1243033
• Replicon: chromosome
• Accession: NZ_CP010444
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: SH07_RS06095
• Protein ID: WP_001290581.1
• Coordinates: 1242415..1242633 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: SH07_RS06100
• Protein ID: WP_000344800.1
• Coordinates: 1242659..1243033 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH07_RS06065	1237704..1238276	+	573	WP_000779842.1	YbaY family lipoprotein	-
SH07_RS06070	1238307..1238618	-	312	WP_000409911.1	MGMT family protein	-
SH07_RS06075	1238997..1239350	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
SH07_RS06080	1239392..1240942	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
SH07_RS06085	1241106..1241576	-	471	WP_000136192.1	YlaC family protein	-
SH07_RS06090	1241692..1242243	-	552	WP_000102564.1	maltose O-acetyltransferase	-
SH07_RS06095	1242415..1242633	-	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
SH07_RS06100	1242659..1243033	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
SH07_RS06105	1243579..1246728	-	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
SH07_RS06110	1246751..1247944	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T52661 WP_001290581.1 NZ_CP010444:c1242633-1242415 [Escherichia coli K-12]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T52661 NZ_CP010444:c1242633-1242415 [Escherichia coli K-12]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT52661 WP_000344800.1 NZ_CP010444:c1243033-1242659 [Escherichia coli K-12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT52661 NZ_CP010444:c1243033-1242659 [Escherichia coli K-12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5