Detailed information of TA system TA052660

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1025746..1026425
• Replicon: chromosome
• Accession: NZ_CP010444
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: SH07_RS04980
• Protein ID: WP_000854672.1
• Coordinates: 1025746..1026087 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: SH07_RS04985
• Protein ID: WP_000070395.1
• Coordinates: 1026108..1026425 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH07_RS04955	1021023..1021424	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
SH07_RS04960	1021463..1022518	-	1056	WP_000749863.1	phosphoporin PhoE	-
SH07_RS04965	1022806..1023909	+	1104	WP_001285288.1	glutamate 5-kinase	-
SH07_RS04970	1023921..1025174	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
SH07_RS04980	1025746..1026087	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
SH07_RS04985	1026108..1026425	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
SH07_RS23430	1026444..1026665	-	222	WP_000691994.1	DUF987 domain-containing protein	-
SH07_RS04990	1026674..1027150	-	477	WP_000811693.1	RadC family protein	-
SH07_RS04995	1027166..1027624	-	459	WP_000211838.1	antirestriction protein	-
SH07_RS05000	1027722..1027961	-	240	WP_000194654.1	DUF905 domain-containing protein	-
SH07_RS05005	1028038..1028505	-	468	WP_001547765.1	hypothetical protein	-
SH07_RS25395	1028528..1028971	-	444	WP_000824223.1	hypothetical protein	-
SH07_RS25400	1028971..1029198	-	228	WP_001548158.1	hypothetical protein	-
SH07_RS25405	1029194..1029385	-	192	Protein_928	DeoR family transcriptional regulator	-
SH07_RS05020	1029602..1030423	-	822	WP_000197389.1	DUF945 domain-containing protein	-
SH07_RS05025	1030515..1031378	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1025526..1044389	18863

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T52660 WP_000854672.1 NZ_CP010444:c1026087-1025746 [Escherichia coli K-12]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T52660 NZ_CP010444:c1026087-1025746 [Escherichia coli K-12]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT52660 WP_000070395.1 NZ_CP010444:c1026425-1026108 [Escherichia coli K-12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT52660 NZ_CP010444:c1026425-1026108 [Escherichia coli K-12]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684