Detailed information of TA system TA052653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 652082..652896
• Replicon: chromosome
• Accession: NZ_CP010444
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: SH07_RS03175
• Protein ID: WP_001054376.1
• Coordinates: 652639..652896 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: SH07_RS03170
• Protein ID: WP_001309181.1
• Coordinates: 652082..652627 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH07_RS03150	647773..649086	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
SH07_RS03155	649098..649376	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
SH07_RS03160	649373..650494	-	1122	WP_000010829.1	M42 family metallopeptidase	-
SH07_RS25855	650739..650855	-	117	Protein_573	VOC family protein	-
SH07_RS24830	650893..651111	-	219	Protein_574	hypothetical protein	-
SH07_RS03165	651280..652026	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
SH07_RS03170	652082..652627	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
SH07_RS03175	652639..652896	-	258	WP_001054376.1	hypothetical protein	Toxin
SH07_RS25340	653273..653518	-	246	Protein_578	GNAT family N-acetyltransferase	-
SH07_RS03190	653634..654874	+	1241	Protein_579	helicase YjhR	-
SH07_RS03195	655457..656437	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
SH07_RS03200	656502..657608	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T52653 WP_001054376.1 NZ_CP010444:c652896-652639 [Escherichia coli K-12]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T52653 NZ_CP010444:c652896-652639 [Escherichia coli K-12]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT52653 WP_001309181.1 NZ_CP010444:c652627-652082 [Escherichia coli K-12]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT52653 NZ_CP010444:c652627-652082 [Escherichia coli K-12]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8