Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2819132..2819963 Replicon chromosome
Accession NZ_CP010443
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag SH04_RS14065 Protein ID WP_000854814.1
Coordinates 2819589..2819963 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag SH04_RS14060 Protein ID WP_001285584.1
Coordinates 2819132..2819500 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH04_RS14045 2816799..2818331 + 1533 WP_001350525.1 hypothetical protein -
SH04_RS25125 2818328..2818774 + 447 WP_000187523.1 RadC family protein -
SH04_RS14055 2818837..2819058 + 222 WP_000692323.1 DUF987 domain-containing protein -
SH04_RS14060 2819132..2819500 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
SH04_RS14065 2819589..2819963 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
SH04_RS14070 2819960..2820154 + 195 WP_000988600.1 hypothetical protein -
SH04_RS25815 2820167..2820280 + 114 WP_001161660.1 DUF957 domain-containing protein -
SH04_RS24190 2820569..2820697 - 129 Protein_2654 transposase domain-containing protein -
SH04_RS14080 2820769..2820951 + 183 WP_001016348.1 hypothetical protein -
SH04_RS14085 2821052..2821381 - 330 WP_000450409.1 DUF496 family protein -
SH04_RS14090 2821553..2822611 - 1059 WP_001200891.1 FUSC family protein -
SH04_RS14095 2822809..2823282 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
SH04_RS14100 2823401..2824567 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T52640 WP_000854814.1 NZ_CP010443:2819589-2819963 [Escherichia coli K-12]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T52640 NZ_CP010443:2819589-2819963 [Escherichia coli K-12]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT52640 WP_001285584.1 NZ_CP010443:2819132-2819500 [Escherichia coli K-12]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT52640 NZ_CP010443:2819132-2819500 [Escherichia coli K-12]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References