Detailed information of TA system TA052619

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1022975..1023654
• Replicon: chromosome
• Accession: NZ_CP010443
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: SH04_RS04975
• Protein ID: WP_000854672.1
• Coordinates: 1022975..1023316 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: SH04_RS04980
• Protein ID: WP_000070395.1
• Coordinates: 1023337..1023654 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH04_RS04950	1018252..1018653	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
SH04_RS04955	1018692..1019747	-	1056	WP_000749863.1	phosphoporin PhoE	-
SH04_RS04960	1020035..1021138	+	1104	WP_001285288.1	glutamate 5-kinase	-
SH04_RS04965	1021150..1022403	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
SH04_RS04975	1022975..1023316	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
SH04_RS04980	1023337..1023654	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
SH04_RS23435	1023673..1023894	-	222	WP_000691994.1	DUF987 domain-containing protein	-
SH04_RS04985	1023903..1024379	-	477	WP_000811693.1	RadC family protein	-
SH04_RS04990	1024395..1024853	-	459	WP_000211838.1	antirestriction protein	-
SH04_RS04995	1024951..1025190	-	240	WP_000194654.1	DUF905 domain-containing protein	-
SH04_RS05000	1025267..1025734	-	468	WP_001547765.1	hypothetical protein	-
SH04_RS25385	1025757..1026200	-	444	WP_000824223.1	hypothetical protein	-
SH04_RS25390	1026200..1026427	-	228	WP_001548158.1	hypothetical protein	-
SH04_RS25395	1026423..1026614	-	192	Protein_926	DeoR family transcriptional regulator	-
SH04_RS05015	1026831..1027652	-	822	WP_000197389.1	DUF945 domain-containing protein	-
SH04_RS05020	1027744..1028607	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	982037..1056668	74631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T52619 WP_000854672.1 NZ_CP010443:c1023316-1022975 [Escherichia coli K-12]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T52619 NZ_CP010443:c1023316-1022975 [Escherichia coli K-12]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT52619 WP_000070395.1 NZ_CP010443:c1023654-1023337 [Escherichia coli K-12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT52619 NZ_CP010443:c1023654-1023337 [Escherichia coli K-12]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684