Detailed information of TA system TA052599

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2872704..2873535
• Replicon: chromosome
• Accession: NZ_CP010442
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: SH06_RS14385
• Protein ID: WP_000854814.1
• Coordinates: 2873161..2873535 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: SH06_RS14380
• Protein ID: WP_001285584.1
• Coordinates: 2872704..2873072 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH06_RS14365	2870371..2871903	+	1533	WP_001350525.1	hypothetical protein	-
SH06_RS25480	2871900..2872346	+	447	WP_000187523.1	RadC family protein	-
SH06_RS14375	2872409..2872630	+	222	WP_000692323.1	DUF987 domain-containing protein	-
SH06_RS14380	2872704..2873072	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
SH06_RS14385	2873161..2873535	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
SH06_RS14390	2873532..2873726	+	195	WP_000988600.1	hypothetical protein	-
SH06_RS26215	2873739..2873852	+	114	WP_001161660.1	DUF957 domain-containing protein	-
SH06_RS24535	2874141..2874269	-	129	Protein_2714	transposase domain-containing protein	-
SH06_RS14400	2874341..2874523	+	183	WP_001016348.1	hypothetical protein	-
SH06_RS14405	2874624..2874953	-	330	WP_000450409.1	DUF496 family protein	-
SH06_RS14410	2875125..2876183	-	1059	WP_001200891.1	FUSC family protein	-
SH06_RS14415	2876381..2876854	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
SH06_RS14420	2876973..2878139	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T52599 WP_000854814.1 NZ_CP010442:2873161-2873535 [Escherichia coli K-12]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T52599 NZ_CP010442:2873161-2873535 [Escherichia coli K-12]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT52599 WP_001285584.1 NZ_CP010442:2872704-2873072 [Escherichia coli K-12]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT52599 NZ_CP010442:2872704-2873072 [Escherichia coli K-12]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6