Detailed information of TA system TA052570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 639902..640527
• Replicon: chromosome
• Accession: NZ_CP010442
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SH06_RS03100
• Protein ID: WP_000911324.1
• Coordinates: 640129..640527 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: SH06_RS03095
• Protein ID: WP_000450532.1
• Coordinates: 639902..640129 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH06_RS03095	639902..640129	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
SH06_RS03100	640129..640527	+	399	WP_000911324.1	tRNA(fMet)-specific endonuclease VapC	Toxin
SH06_RS03105	640536..642689	-	2154	WP_000009350.1	type IV conjugative transfer system coupling protein TraD	-
SH06_RS25685	642942..643673	-	732	WP_000850422.1	complement resistance protein TraT	-
SH06_RS25690	643698..644219	-	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	fimB / fimE / fimA / fimI / fimC / fimD / fimF / fimG / fimH	614676..729232	114556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T52570 WP_000911324.1 NZ_CP010442:640129-640527 [Escherichia coli K-12]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T52570 NZ_CP010442:640129-640527 [Escherichia coli K-12]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGGTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCTTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT52570 WP_000450532.1 NZ_CP010442:639902-640129 [Escherichia coli K-12]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT52570 NZ_CP010442:639902-640129 [Escherichia coli K-12]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5