Detailed information of TA system TA052529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 650086..650900
• Replicon: chromosome
• Accession: NZ_CP010441
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: SH03_RS03175
• Protein ID: WP_001054376.1
• Coordinates: 650643..650900 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: SH03_RS03170
• Protein ID: WP_001309181.1
• Coordinates: 650086..650631 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH03_RS03150	645777..647090	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
SH03_RS03155	647102..647380	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
SH03_RS03160	647377..648498	-	1122	WP_000010829.1	M42 family metallopeptidase	-
SH03_RS25855	648743..648859	-	117	Protein_572	VOC family protein	-
SH03_RS24840	648897..649115	-	219	Protein_573	hypothetical protein	-
SH03_RS03165	649284..650030	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
SH03_RS03170	650086..650631	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
SH03_RS03175	650643..650900	-	258	WP_001054376.1	hypothetical protein	Toxin
SH03_RS25340	651277..651522	-	246	Protein_577	GNAT family N-acetyltransferase	-
SH03_RS03190	651638..652878	+	1241	Protein_578	helicase YjhR	-
SH03_RS03195	653461..654441	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
SH03_RS03200	654506..655612	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T52529 WP_001054376.1 NZ_CP010441:c650900-650643 [Escherichia coli K-12]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T52529 NZ_CP010441:c650900-650643 [Escherichia coli K-12]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT52529 WP_001309181.1 NZ_CP010441:c650631-650086 [Escherichia coli K-12]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT52529 NZ_CP010441:c650631-650086 [Escherichia coli K-12]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8