52519

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 3527391..3528058 Replicon chromosome
Accession NZ_CP010440
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag SH08_RS17460 Protein ID WP_001094400.1
Coordinates 3527729..3528058 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag SH08_RS17455 Protein ID WP_000072690.1
Coordinates 3527391..3527708 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH08_RS17435 3522443..3523452 - 1010 Protein_3304 arsenic transporter -
SH08_RS17440 3523594..3525297 + 1704 WP_000896263.1 hypothetical protein -
SH08_RS24525 3525869..3526092 + 224 Protein_3306 DUF905 family protein -
SH08_RS17445 3526195..3526653 + 459 WP_000211841.1 antirestriction protein -
SH08_RS17450 3526662..3527144 + 483 WP_001407480.1 RadC family protein -
SH08_RS24530 3527153..3527353 + 201 WP_001395452.1 DUF987 domain-containing protein -
SH08_RS17455 3527391..3527708 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
SH08_RS17460 3527729..3528058 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
SH08_RS23095 3528422..3533002 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T52519 WP_001094400.1 NZ_CP010440:3527729-3528058 [Escherichia coli K-12]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T52519 NZ_CP010440:3527729-3528058 [Escherichia coli K-12]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT52519 WP_000072690.1 NZ_CP010440:3527391-3527708 [Escherichia coli K-12]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT52519 NZ_CP010440:3527391-3527708 [Escherichia coli K-12]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
AlphaFold DB P52141

References