Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1240623..1241241 Replicon chromosome
Accession NZ_CP010440
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag SH08_RS06110 Protein ID WP_001291435.1
Coordinates 1240623..1240841 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag SH08_RS06115 Protein ID WP_000344800.1
Coordinates 1240867..1241241 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH08_RS06080 1235912..1236484 + 573 WP_000779842.1 YbaY family lipoprotein -
SH08_RS06085 1236515..1236826 - 312 WP_000409911.1 MGMT family protein -
SH08_RS06090 1237205..1237558 + 354 WP_000878140.1 DUF1428 domain-containing protein -
SH08_RS06095 1237600..1239150 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
SH08_RS06100 1239314..1239784 - 471 WP_000136192.1 YlaC family protein -
SH08_RS06105 1239900..1240451 - 552 WP_000102564.1 maltose O-acetyltransferase -
SH08_RS06110 1240623..1240841 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
SH08_RS06115 1240867..1241241 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
SH08_RS06120 1241787..1244936 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
SH08_RS06125 1244959..1246152 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T52497 WP_001291435.1 NZ_CP010440:c1240841-1240623 [Escherichia coli K-12]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T52497 NZ_CP010440:c1240841-1240623 [Escherichia coli K-12]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT52497 WP_000344800.1 NZ_CP010440:c1241241-1240867 [Escherichia coli K-12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT52497 NZ_CP010440:c1241241-1240867 [Escherichia coli K-12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References