Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3774406..3775060 Replicon chromosome
Accession NZ_CP010439
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag SH02_RS18700 Protein ID WP_000244777.1
Coordinates 3774406..3774813 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID -
Locus tag SH02_RS18705 Protein ID WP_046377553.1
Coordinates 3774830..3775060 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH02_RS18680 3770363..3772096 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
SH02_RS18685 3772102..3772812 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
SH02_RS18690 3772837..3773733 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
SH02_RS18695 3773845..3774366 + 522 WP_001055874.1 flavodoxin FldB -
SH02_RS18700 3774406..3774813 - 408 WP_000244777.1 toxin CptA Toxin
SH02_RS18705 3774830..3775060 - 231 WP_046377553.1 FAD assembly factor SdhE Antitoxin
SH02_RS18710 3775303..3776283 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
SH02_RS18715 3776479..3777138 - 660 WP_000250274.1 hemolysin III family protein -
SH02_RS18720 3777302..3777613 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
SH02_RS18725 3777658..3779091 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
SH02_RS18730 3779148..3779891 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-76)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T52483 WP_000244777.1 NZ_CP010439:c3774813-3774406 [Escherichia coli K-12]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T52483 NZ_CP010439:c3774813-3774406 [Escherichia coli K-12]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 9168.56 Da        Isoelectric Point: 4.6388

>AT52483 WP_046377553.1 NZ_CP010439:c3775060-3774830 [Escherichia coli K-12]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLI

Download         Length: 231 bp

>AT52483 NZ_CP010439:c3775060-3774830 [Escherichia coli K-12]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7


Antitoxin

Source ID Structure

References