52481

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 3510766..3511433 Replicon chromosome
Accession NZ_CP010439
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag SH02_RS17420 Protein ID WP_001094400.1
Coordinates 3511104..3511433 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag SH02_RS17415 Protein ID WP_000072690.1
Coordinates 3510766..3511083 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH02_RS17395 3505818..3506827 - 1010 Protein_3294 arsenic transporter -
SH02_RS17400 3506969..3508672 + 1704 WP_000896263.1 hypothetical protein -
SH02_RS24475 3509244..3509467 + 224 Protein_3296 DUF905 family protein -
SH02_RS17405 3509570..3510028 + 459 WP_000211841.1 antirestriction protein -
SH02_RS17410 3510037..3510519 + 483 WP_001407480.1 RadC family protein -
SH02_RS24480 3510528..3510728 + 201 WP_001395452.1 DUF987 domain-containing protein -
SH02_RS17415 3510766..3511083 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
SH02_RS17420 3511104..3511433 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
SH02_RS23050 3511797..3516377 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T52481 WP_001094400.1 NZ_CP010439:3511104-3511433 [Escherichia coli K-12]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T52481 NZ_CP010439:3511104-3511433 [Escherichia coli K-12]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT52481 WP_000072690.1 NZ_CP010439:3510766-3511083 [Escherichia coli K-12]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT52481 NZ_CP010439:3510766-3511083 [Escherichia coli K-12]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
AlphaFold DB P52141

References