Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2249767..2250405 Replicon chromosome
Accession NZ_CP010439
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag SH02_RS11205 Protein ID WP_000813794.1
Coordinates 2249767..2249943 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag SH02_RS11210 Protein ID WP_001270286.1
Coordinates 2249989..2250405 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH02_RS11185 2245386..2246600 - 1215 WP_001326689.1 BenE family transporter YdcO -
SH02_RS11190 2246653..2247189 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
SH02_RS11195 2247262..2249223 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
SH02_RS11200 2249315..2249545 - 231 WP_000494244.1 YncJ family protein -
SH02_RS11205 2249767..2249943 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
SH02_RS11210 2249989..2250405 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
SH02_RS11215 2250484..2251890 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
SH02_RS11220 2252135..2253280 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
SH02_RS11225 2253298..2254311 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
SH02_RS11230 2254312..2255253 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T52467 WP_000813794.1 NZ_CP010439:2249767-2249943 [Escherichia coli K-12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T52467 NZ_CP010439:2249767-2249943 [Escherichia coli K-12]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT52467 WP_001270286.1 NZ_CP010439:2249989-2250405 [Escherichia coli K-12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT52467 NZ_CP010439:2249989-2250405 [Escherichia coli K-12]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References