52456

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 469431..469928 Replicon chromosome
Accession NZ_CP010439
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag SH02_RS02230 Protein ID WP_001173343.1
Coordinates 469755..469928 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag SH02_RS02225 Protein ID WP_000398619.1
Coordinates 469431..469727 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH02_RS02205 466162..466881 - 720 WP_000611288.1 two-component system response regulator DcuR -
SH02_RS02210 466878..468509 - 1632 WP_001216477.1 sensor histidine kinase -
SH02_RS02215 468690..468920 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
SH02_RS02220 468932..469204 + 273 WP_000405651.1 N-acetyltransferase -
SH02_RS02225 469431..469727 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
SH02_RS02230 469755..469928 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
SH02_RS02235 470047..471564 - 1518 WP_001295074.1 lysine--tRNA ligase -
SH02_RS02240 471801..473258 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T52456 WP_001173343.1 NZ_CP010439:469755-469928 [Escherichia coli K-12]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T52456 NZ_CP010439:469755-469928 [Escherichia coli K-12]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT52456 WP_000398619.1 NZ_CP010439:469431-469727 [Escherichia coli K-12]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT52456 NZ_CP010439:469431-469727 [Escherichia coli K-12]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References