Detailed information of TA system TA052450

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3789592..3790246
• Replicon: chromosome
• Accession: NZ_CP010438
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: SH05_RS18745
• Protein ID: WP_000244777.1
• Coordinates: 3789592..3789999 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: SH05_RS18750
• Protein ID: WP_046377553.1
• Coordinates: 3790016..3790246 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH05_RS18725	3785549..3787282	-	1734	WP_000813200.1	single-stranded-DNA-specific exonuclease RecJ	-
SH05_RS18730	3787288..3787998	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
SH05_RS18735	3788023..3788919	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
SH05_RS18740	3789031..3789552	+	522	WP_001055874.1	flavodoxin FldB	-
SH05_RS18745	3789592..3789999	-	408	WP_000244777.1	toxin CptA	Toxin
SH05_RS18750	3790016..3790246	-	231	WP_046377553.1	FAD assembly factor SdhE	Antitoxin
SH05_RS18755	3790489..3791469	+	981	WP_000886062.1	tRNA-modifying protein YgfZ	-
SH05_RS18760	3791665..3792324	-	660	WP_000250274.1	hemolysin III family protein	-
SH05_RS18765	3792488..3792799	-	312	WP_001182957.1	N(4)-acetylcytidine aminohydrolase	-
SH05_RS18770	3792844..3794277	+	1434	WP_001336277.1	6-phospho-beta-glucosidase BglA	-
SH05_RS18775	3794334..3795077	-	744	WP_000951964.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-76)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T52450 WP_000244777.1 NZ_CP010438:c3789999-3789592 [Escherichia coli K-12]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T52450 NZ_CP010438:c3789999-3789592 [Escherichia coli K-12]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9168.56 Da
• Isoelectric Point: 4.6388

>AT52450 WP_046377553.1 NZ_CP010438:c3790246-3790016 [Escherichia coli K-12]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLI

Nucleotide

• Length: 231 bp

>AT52450 NZ_CP010438:c3790246-3790016 [Escherichia coli K-12]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATTTAG

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7