Detailed information of TA system TA052422

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1025079..1025758
• Replicon: chromosome
• Accession: NZ_CP010438
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: SH05_RS04995
• Protein ID: WP_000854672.1
• Coordinates: 1025079..1025420 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: SH05_RS05000
• Protein ID: WP_000070395.1
• Coordinates: 1025441..1025758 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH05_RS04970	1020356..1020757	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
SH05_RS04975	1020796..1021851	-	1056	WP_000749863.1	phosphoporin PhoE	-
SH05_RS04980	1022139..1023242	+	1104	WP_001285288.1	glutamate 5-kinase	-
SH05_RS04985	1023254..1024507	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
SH05_RS04995	1025079..1025420	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
SH05_RS05000	1025441..1025758	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
SH05_RS23545	1025777..1025998	-	222	WP_000691994.1	DUF987 domain-containing protein	-
SH05_RS05005	1026007..1026483	-	477	WP_000811693.1	RadC family protein	-
SH05_RS05010	1026499..1026957	-	459	WP_000211838.1	antirestriction protein	-
SH05_RS05015	1027055..1027294	-	240	WP_000194654.1	DUF905 domain-containing protein	-
SH05_RS05020	1027371..1027838	-	468	WP_001547765.1	hypothetical protein	-
SH05_RS25535	1027861..1028304	-	444	WP_000824223.1	hypothetical protein	-
SH05_RS25540	1028304..1028531	-	228	WP_001548158.1	hypothetical protein	-
SH05_RS25545	1028527..1028718	-	192	Protein_929	DeoR family transcriptional regulator	-
SH05_RS05035	1028935..1029756	-	822	WP_000197389.1	DUF945 domain-containing protein	-
SH05_RS05040	1029848..1030711	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T52422 WP_000854672.1 NZ_CP010438:c1025420-1025079 [Escherichia coli K-12]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T52422 NZ_CP010438:c1025420-1025079 [Escherichia coli K-12]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT52422 WP_000070395.1 NZ_CP010438:c1025758-1025441 [Escherichia coli K-12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT52422 NZ_CP010438:c1025758-1025441 [Escherichia coli K-12]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684