Detailed information of TA system TA052399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 156435..157069
• Replicon: chromosome
• Accession: NZ_CP010431
• Organism: Pandoraea sputorum strain DSM 21091

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A239S680
• Locus tag: NA29_RS00660
• Protein ID: WP_039394568.1
• Coordinates: 156650..157069 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: NA29_RS00655
• Protein ID: WP_150660872.1
• Coordinates: 156435..156635 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NA29_RS00635	151444..151845	+	402	WP_039394559.1	PaaI family thioesterase	-
NA29_RS00640	151890..152735	+	846	WP_039394562.1	patatin-like phospholipase family protein	-
NA29_RS00645	152820..153830	-	1011	WP_039394564.1	D-2-hydroxyacid dehydrogenase family protein	-
NA29_RS00650	154352..156169	+	1818	WP_095178504.1	phosphoenolpyruvate carboxykinase (GTP)	-
NA29_RS00655	156435..156635	+	201	WP_150660872.1	DNA-binding protein	Antitoxin
NA29_RS00660	156650..157069	+	420	WP_039394568.1	type II toxin-antitoxin system VapC family toxin	Toxin
NA29_RS00665	157093..157725	-	633	WP_039394571.1	response regulator transcription factor	-
NA29_RS00670	157722..159110	-	1389	WP_039394574.1	cache domain-containing protein	-
NA29_RS00675	159541..161607	+	2067	WP_039394577.1	carbon starvation protein A	-
NA29_RS00680	161646..161843	+	198	WP_039394580.1	YbdD/YjiX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15928.45 Da
• Isoelectric Point: 10.4402

>T52399 WP_039394568.1 NZ_CP010431:156650-157069 [Pandoraea sputorum]
MYLLDTNVISESRKRGGGDEGVQRFMRMTDRRKAPRFLSVITMGELQRGVKVLRHRNDVRQASLIESWLAQLRLDYANDI
LPVDQEICNVWARMRVPNAAHPIDKLIAATALVHKLIVVTRNVKDFSNTGVEVYNPFQP

Nucleotide

• Length: 420 bp

>T52399 NZ_CP010431:156650-157069 [Pandoraea sputorum]
ATGTACTTACTGGATACCAACGTCATTAGCGAAAGCCGAAAGAGGGGAGGCGGCGATGAAGGGGTTCAGCGCTTCATGCG
AATGACCGATCGGCGTAAGGCACCTCGCTTTCTATCCGTCATTACGATGGGCGAGCTGCAACGTGGGGTTAAGGTGCTCC
GGCACAGAAACGATGTTCGACAGGCATCGCTAATCGAGTCTTGGTTGGCCCAACTAAGACTCGATTACGCCAACGACATA
CTGCCGGTCGATCAGGAGATCTGCAACGTTTGGGCACGCATGCGCGTGCCCAACGCAGCGCACCCAATCGATAAATTGAT
TGCGGCCACAGCGTTGGTGCACAAGTTGATCGTCGTCACACGCAACGTCAAAGATTTTTCCAACACAGGTGTGGAGGTGT
ACAACCCGTTTCAGCCATAG

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7520.68 Da
• Isoelectric Point: 4.4155

>AT52399 WP_150660872.1 NZ_CP010431:156435-156635 [Pandoraea sputorum]
MMLIEGIDEQLMRSIESRAAEGGRTPEEEVLMILDRMARVPRFRSLGEALRAMPNVGLDSDFERIN

Nucleotide

• Length: 201 bp

>AT52399 NZ_CP010431:156435-156635 [Pandoraea sputorum]
ATGATGCTGATTGAAGGTATCGACGAGCAACTGATGCGGTCGATTGAGTCGAGAGCTGCCGAGGGTGGCAGAACGCCGGA
AGAGGAAGTTCTGATGATTTTGGATCGTATGGCGAGGGTGCCGAGATTCCGGTCGTTGGGAGAAGCGCTGCGAGCCATGC
CGAATGTCGGTCTCGATAGCGACTTCGAGCGGATCAACTAA

Structures

Toxin

• AlphaFold DB: A0A239S680