Detailed information of TA system TA052393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 269687..270570
• Replicon: plasmid pAcX50f
• Accession: NZ_CP010421
• Organism: Azotobacter chroococcum NCIMB 8003

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A0C4WT88
• Locus tag: Achr_RS23155
• Protein ID: WP_040107448.1
• Coordinates: 269687..270124 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: PrcA
• Uniprot ID: A0A0C4WY60
• Locus tag: Achr_RS23160
• Protein ID: WP_040107449.1
• Coordinates: 270121..270570 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Achr_RS23125	265326..266207	-	882	WP_040107442.1	LysR family transcriptional regulator	-
Achr_RS23130	266338..267564	+	1227	WP_040107443.1	YbfB/YjiJ family MFS transporter	-
Achr_RS23135	267899..268171	-	273	WP_040107444.1	HU family DNA-binding protein	-
Achr_RS23140	268303..268782	-	480	WP_040107445.1	hypothetical protein	-
Achr_RS23145	269031..269426	-	396	WP_040107446.1	hypothetical protein	-
Achr_RS23150	269454..269636	-	183	WP_040107447.1	hypothetical protein	-
Achr_RS23155	269687..270124	-	438	WP_040107448.1	RES family NAD+ phosphorylase	Toxin
Achr_RS23160	270121..270570	-	450	WP_040107449.1	DUF2384 domain-containing protein	Antitoxin
Achr_RS23165	270784..271272	+	489	WP_040107450.1	hypothetical protein	-
Achr_RS23170	271570..272694	+	1125	WP_040107529.1	Fic family protein	-
Achr_RS23175	272849..273577	-	729	WP_040107451.1	NADPH-dependent F420 reductase	-
Achr_RS23180	273606..274997	-	1392	WP_131254715.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..311724	311724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-140)

Antitoxin

• Antitoxin Xre (23-146)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15880.25 Da
• Isoelectric Point: 5.1577

>T52393 WP_040107448.1 NZ_CP010421:c270124-269687 [Azotobacter chroococcum NCIMB 8003]
VILWRISAYADLSGIGGLRASGRWHHLGRPVVYAADSPAGAMLEVLVHLEIDPEDFPTTLQLIRVELPDLVSRAELPPLP
EHWKSDPEVTRAMGDRFLDGRSALLLPVPSAIIPCTTNYLFNPAHPEASGASIQAEKFPLDSRLI

Nucleotide

• Length: 438 bp

>T52393 NZ_CP010421:c270124-269687 [Azotobacter chroococcum NCIMB 8003]
GTGATTCTGTGGCGGATCAGCGCCTACGCGGACCTCAGTGGAATCGGTGGCCTGCGTGCCAGTGGTCGCTGGCACCACCT
TGGTAGGCCGGTCGTTTACGCGGCGGACAGCCCGGCCGGTGCGATGCTGGAGGTGCTGGTCCATCTGGAAATCGACCCCG
AGGACTTCCCCACGACGCTCCAGTTGATCCGCGTCGAGCTTCCAGACCTGGTTTCGCGGGCCGAGCTTCCGCCGCTGCCG
GAGCACTGGAAATCCGACCCGGAGGTCACGCGCGCCATGGGTGATCGCTTTCTGGATGGGCGAAGCGCTCTGCTGCTCCC
GGTGCCGAGCGCCATCATTCCCTGCACGACCAACTACCTGTTCAACCCGGCGCACCCCGAGGCCAGTGGGGCGAGCATCC
AGGCAGAGAAATTCCCCCTCGATTCCCGCCTCATCTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17026.65 Da
• Isoelectric Point: 6.7345

>AT52393 WP_040107449.1 NZ_CP010421:c270570-270121 [Azotobacter chroococcum NCIMB 8003]
MLAEVLRDHGYTAYRARLQSLLDIPESATDFEIHTRITKGFPATRLVQLTEAGVVTPLERDQIIPLRTLKSRIERDQPLT
VEESDRFFRTAHIMAMAEAVFGDREKAKRWLSKPKERFSGLTPMQMLTTQQGTSQVEEMLLQIVEGLAL

Nucleotide

• Length: 450 bp

>AT52393 NZ_CP010421:c270570-270121 [Azotobacter chroococcum NCIMB 8003]
ATGCTTGCTGAAGTCCTGCGTGACCATGGCTACACCGCATACCGGGCGCGTCTTCAAAGCTTGCTGGACATTCCGGAGTC
GGCAACTGACTTCGAGATCCACACCCGGATCACCAAGGGCTTTCCTGCAACCCGCCTGGTGCAGCTGACCGAGGCCGGAG
TGGTTACGCCGCTGGAGCGCGACCAGATCATCCCCTTGCGAACCCTCAAGAGTCGCATCGAGCGGGATCAGCCGTTGACC
GTGGAGGAAAGCGACCGGTTTTTTCGCACTGCCCACATCATGGCAATGGCCGAGGCTGTCTTCGGTGACCGCGAGAAGGC
CAAACGCTGGCTCTCCAAACCCAAGGAGCGGTTTTCCGGTCTGACGCCCATGCAGATGCTCACCACCCAGCAGGGCACCA
GCCAAGTAGAGGAAATGCTGCTGCAGATCGTCGAAGGTCTGGCCCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0C4WT88

Antitoxin

• AlphaFold DB: A0A0C4WY60