Detailed information of TA system TA052392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 226286..226929
• Replicon: plasmid pAcX50f
• Accession: NZ_CP010421
• Organism: Azotobacter chroococcum NCIMB 8003

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: Achr_RS22990
• Protein ID: WP_040107520.1
• Coordinates: 226286..226684 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A0C4WT59
• Locus tag: Achr_RS22995
• Protein ID: WP_040107521.1
• Coordinates: 226699..226929 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Achr_RS22970	221421..222563	-	1143	WP_040107421.1	hypothetical protein	-
Achr_RS22975	222565..223836	-	1272	WP_040107422.1	restriction endonuclease subunit S	-
Achr_RS22980	223833..225389	-	1557	WP_040107423.1	type I restriction-modification system subunit M	-
Achr_RS22985	225701..225907	-	207	WP_040107424.1	helix-turn-helix domain-containing protein	-
Achr_RS22990	226286..226684	-	399	WP_040107520.1	type II toxin-antitoxin system VapC family toxin	Toxin
Achr_RS22995	226699..226929	-	231	WP_040107521.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Achr_RS23000	227107..228867	-	1761	WP_052264100.1	DUF4942 domain-containing protein	-
Achr_RS23005	229000..229422	-	423	WP_052264101.1	hypothetical protein	-
Achr_RS23010	229799..230002	-	204	WP_040107425.1	hypothetical protein	-
Achr_RS23015	230095..230859	-	765	WP_040107426.1	PRTRC system ThiF family protein	-
Achr_RS23020	230856..231569	-	714	WP_052264102.1	hypothetical protein	-
Achr_RS25680	231556..231834	-	279	WP_040107427.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..311724	311724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14384.55 Da
• Isoelectric Point: 6.4700

>T52392 WP_040107520.1 NZ_CP010421:c226684-226286 [Azotobacter chroococcum NCIMB 8003]
MLDTCICSFIMRELPESVLQRLAAEVSQGNRIVISAVTYAEMRYGQIGKKASARHKTLVDEFVKRLDAVLSWDQCAVDAT
VEVMQALTKAGTPIGHNDTAIAGHAIASGCTLVTNNVREFGRVPGLVHEDWV

Nucleotide

• Length: 399 bp

>T52392 NZ_CP010421:c226684-226286 [Azotobacter chroococcum NCIMB 8003]
ATGCTCGATACCTGCATTTGCTCGTTCATCATGCGGGAACTCCCGGAGTCGGTTTTACAACGGCTGGCGGCAGAGGTTAG
CCAAGGCAATCGAATCGTTATTTCTGCCGTCACCTACGCCGAAATGCGTTATGGCCAGATCGGCAAGAAAGCCTCAGCCA
GGCATAAAACGCTGGTCGATGAATTCGTGAAGCGGCTAGATGCCGTGTTGTCGTGGGATCAATGCGCCGTAGATGCGACC
GTTGAAGTCATGCAAGCGCTGACCAAGGCGGGCACGCCAATCGGCCACAATGACACGGCTATCGCAGGTCATGCGATAGC
CTCCGGCTGCACGCTAGTGACAAACAATGTTCGTGAGTTTGGCCGTGTACCTGGCCTTGTACATGAGGACTGGGTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8513.66 Da
• Isoelectric Point: 5.0338

>AT52392 WP_040107521.1 NZ_CP010421:c226929-226699 [Azotobacter chroococcum NCIMB 8003]
MRTVSIFRNGKNQAIRLPNDMAYEGVGELEITREGDVITLRPVRPTWQSLAGVPKADADFLQERPTVVGDEGRFNL

Nucleotide

• Length: 231 bp

>AT52392 NZ_CP010421:c226929-226699 [Azotobacter chroococcum NCIMB 8003]
ATGCGTACCGTCTCTATTTTCAGGAACGGCAAGAATCAGGCGATTCGACTGCCGAACGACATGGCCTATGAAGGCGTCGG
GGAGCTGGAGATTACGCGCGAAGGTGACGTAATCACGCTTCGTCCGGTACGGCCAACGTGGCAGTCCCTAGCTGGCGTGC
CGAAAGCCGATGCCGACTTTCTACAAGAGCGCCCGACTGTTGTCGGTGATGAAGGCAGGTTCAATCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0C4WT59