52389

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 190957..191750 Replicon plasmid pAcX50f
Accession NZ_CP010421
Organism Azotobacter chroococcum NCIMB 8003

Toxin (Protein)


Gene name tacT Uniprot ID A0A0C4WM27
Locus tag Achr_RS22850 Protein ID WP_040107406.1
Coordinates 190957..191475 (-) Length 173 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A0C4WT96
Locus tag Achr_RS22855 Protein ID WP_040107407.1
Coordinates 191475..191750 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Achr_RS22835 186089..186466 + 378 WP_144411658.1 hypothetical protein -
Achr_RS24760 186593..187774 + 1182 WP_082045671.1 MFS transporter -
Achr_RS22840 188239..189198 + 960 WP_040107404.1 2-hydroxyacid dehydrogenase -
Achr_RS24765 189406..189855 + 450 WP_082045673.1 hypothetical protein -
Achr_RS22845 189981..190940 + 960 WP_193388246.1 endonuclease -
Achr_RS22850 190957..191475 - 519 WP_040107406.1 GNAT family N-acetyltransferase Toxin
Achr_RS22855 191475..191750 - 276 WP_040107407.1 DUF1778 domain-containing protein Antitoxin
Achr_RS22860 192033..192926 - 894 WP_144411659.1 LysR family transcriptional regulator -
Achr_RS22865 193039..194220 + 1182 WP_040107408.1 serine hydrolase -
Achr_RS22870 194304..195482 + 1179 WP_040107515.1 MFS transporter -
Achr_RS22875 195495..196586 - 1092 WP_040107409.1 MBL fold metallo-hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..311724 311724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(6-90)


Sequences

Toxin        


Download         Length: 173 a.a.        Molecular weight: 18685.46 Da        Isoelectric Point: 9.4747

>T52389 WP_040107406.1 NZ_CP010421:c191475-190957 [Azotobacter chroococcum NCIMB 8003]
MSLTSPQALDASHSLEGFDCGKPSLDDWLMRHARQAQASGSAKTYVVADGVRVAGYYSLTVGQIDTLEAPARVRQGMGAY
PIPVVILARMAVTRQDQGRGIGVGMLQDAIRRTLTIAEQAGVRALLTHPIDEEASRFYLRFGFEASPAREQQLLLLLKDA
RRLLVPPASPRG

Download         Length: 519 bp

>T52389 NZ_CP010421:c191475-190957 [Azotobacter chroococcum NCIMB 8003]
ATGTCGCTGACCTCGCCCCAAGCACTGGACGCCAGCCATTCACTGGAGGGTTTCGATTGCGGCAAACCGAGCCTGGATGA
TTGGCTGATGCGCCATGCCCGGCAGGCGCAGGCCAGCGGTTCGGCCAAGACCTACGTAGTCGCTGACGGCGTGCGCGTCG
CGGGCTACTACAGCCTCACCGTGGGACAGATCGATACCCTGGAAGCTCCCGCTCGTGTCCGCCAAGGCATGGGGGCCTAC
CCCATACCTGTTGTCATCCTGGCGCGGATGGCCGTAACGCGACAGGATCAAGGGCGCGGCATCGGCGTAGGCATGTTGCA
GGATGCGATCCGGCGTACCCTCACCATCGCCGAGCAGGCCGGCGTTCGGGCGCTGTTGACGCATCCGATCGACGAGGAGG
CATCACGCTTCTACCTGCGCTTCGGCTTCGAAGCATCGCCTGCCCGCGAGCAGCAATTGCTGTTGCTGCTCAAGGACGCG
CGTCGGCTGCTTGTTCCGCCGGCGTCCCCCAGGGGATAG

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10046.45 Da        Isoelectric Point: 6.2594

>AT52389 WP_040107407.1 NZ_CP010421:c191750-191475 [Azotobacter chroococcum NCIMB 8003]
MASTIRSARLGLRATPQQEAVLRRAAEVANKSMTDFILDSACQAAEQTLLDQRLFLVSGSQSQALLEMLDRPAQDNPGLK
DLFSRSVPWDK

Download         Length: 276 bp

>AT52389 NZ_CP010421:c191750-191475 [Azotobacter chroococcum NCIMB 8003]
ATGGCAAGTACCATCCGTTCTGCCCGTCTTGGGTTGCGCGCCACGCCGCAGCAGGAAGCGGTGCTGCGCCGCGCGGCCGA
GGTGGCAAACAAATCGATGACCGATTTTATCCTCGACAGTGCCTGTCAGGCCGCCGAGCAGACCTTGCTCGATCAACGCC
TGTTTCTGGTATCCGGCAGCCAGTCCCAAGCTTTACTGGAGATGCTGGACCGGCCTGCCCAGGACAATCCTGGCCTGAAG
GATTTGTTCTCACGATCGGTACCTTGGGACAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C4WM27

Antitoxin

Source ID Structure
AlphaFold DB A0A0C4WT96

References