Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 88777..89395 Replicon plasmid pAcX50f
Accession NZ_CP010421
Organism Azotobacter chroococcum NCIMB 8003

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I4HUC9
Locus tag Achr_RS22320 Protein ID WP_040107493.1
Coordinates 88777..88959 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0C4WXU9
Locus tag Achr_RS22325 Protein ID WP_040107336.1
Coordinates 88991..89395 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Achr_RS22285 84524..84817 - 294 WP_040107331.1 antibiotic biosynthesis monooxygenase -
Achr_RS22290 84831..85625 - 795 WP_040107332.1 SDR family oxidoreductase -
Achr_RS22295 85713..86426 - 714 WP_040107333.1 type 1 glutamine amidotransferase domain-containing protein -
Achr_RS22300 86525..87460 + 936 WP_040107334.1 LysR family transcriptional regulator -
Achr_RS25615 87496..87594 - 99 WP_089170148.1 IS66 family insertion sequence hypothetical protein -
Achr_RS22310 87695..87952 - 258 WP_082045657.1 transposase -
Achr_RS22315 88277..88663 + 387 Protein_95 IS4 family transposase -
Achr_RS22320 88777..88959 + 183 WP_040107493.1 type II toxin-antitoxin system HicA family toxin Toxin
Achr_RS22325 88991..89395 + 405 WP_040107336.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
Achr_RS22330 89521..89784 + 264 WP_040107494.1 hypothetical protein -
Achr_RS22335 90048..91133 + 1086 WP_039807303.1 IS5 family transposase -
Achr_RS22340 91399..92007 + 609 WP_040107337.1 cupin -
Achr_RS22345 92097..93320 - 1224 WP_052264083.1 OprD family porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..311724 311724
- inside IScluster/Tn - - 87496..91133 3637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6800.85 Da        Isoelectric Point: 10.4803

>T52387 WP_040107493.1 NZ_CP010421:88777-88959 [Azotobacter chroococcum NCIMB 8003]
MRSREVIELLEADGWYEVAVKGSHHQFKHPTKPGRVTVPHPKSDLPKGTWQSILKQAGLK

Download         Length: 183 bp

>T52387 NZ_CP010421:88777-88959 [Azotobacter chroococcum NCIMB 8003]
ATGCGAAGTCGGGAAGTGATCGAGCTGCTGGAGGCGGATGGTTGGTACGAAGTGGCGGTGAAAGGTAGTCACCACCAGTT
CAAGCACCCGACCAAGCCGGGGAGGGTGACGGTTCCGCACCCGAAATCGGATCTGCCGAAAGGCACCTGGCAGAGCATCC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14695.64 Da        Isoelectric Point: 4.6292

>AT52387 WP_040107336.1 NZ_CP010421:88991-89395 [Azotobacter chroococcum NCIMB 8003]
MKYPVVIHKEQSSDYGVTVPDVPGCFSAGETMTEALENVREALELHFEGLLADGEPLPQPQEIDVHAANPDYAGGVWALV
DFDVMPYLGKAVRFNATLPEHLLARIDAYVKAHPEQKSRSGFLAEAALRVLQHA

Download         Length: 405 bp

>AT52387 NZ_CP010421:88991-89395 [Azotobacter chroococcum NCIMB 8003]
ATGAAATATCCCGTCGTCATCCACAAAGAGCAGAGCAGCGACTACGGCGTGACCGTGCCGGACGTGCCAGGCTGCTTCTC
GGCCGGCGAGACGATGACCGAAGCGCTGGAGAATGTCCGTGAGGCGCTGGAGTTGCATTTCGAGGGGCTGCTGGCCGATG
GCGAGCCGCTGCCGCAGCCGCAGGAGATCGATGTGCACGCGGCGAACCCGGACTATGCCGGCGGCGTGTGGGCGCTGGTT
GACTTCGATGTGATGCCCTATCTGGGGAAGGCAGTGCGCTTCAACGCCACGCTGCCGGAGCACCTGCTGGCGCGGATCGA
TGCCTACGTGAAGGCCCATCCGGAGCAGAAGAGCCGCTCTGGATTTCTGGCTGAGGCGGCGCTGCGGGTGTTGCAGCACG
CTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I4HUC9

Antitoxin

Source ID Structure
AlphaFold DB A0A0C4WXU9

References