Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | hicAB/HicA-HicB |
Location | 88777..89395 | Replicon | plasmid pAcX50f |
Accession | NZ_CP010421 | ||
Organism | Azotobacter chroococcum NCIMB 8003 |
Toxin (Protein)
Gene name | hicA | Uniprot ID | A0A1I4HUC9 |
Locus tag | Achr_RS22320 | Protein ID | WP_040107493.1 |
Coordinates | 88777..88959 (+) | Length | 61 a.a. |
Antitoxin (Protein)
Gene name | hicB | Uniprot ID | A0A0C4WXU9 |
Locus tag | Achr_RS22325 | Protein ID | WP_040107336.1 |
Coordinates | 88991..89395 (+) | Length | 135 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
Achr_RS22285 | 84524..84817 | - | 294 | WP_040107331.1 | antibiotic biosynthesis monooxygenase | - |
Achr_RS22290 | 84831..85625 | - | 795 | WP_040107332.1 | SDR family oxidoreductase | - |
Achr_RS22295 | 85713..86426 | - | 714 | WP_040107333.1 | type 1 glutamine amidotransferase domain-containing protein | - |
Achr_RS22300 | 86525..87460 | + | 936 | WP_040107334.1 | LysR family transcriptional regulator | - |
Achr_RS25615 | 87496..87594 | - | 99 | WP_089170148.1 | IS66 family insertion sequence hypothetical protein | - |
Achr_RS22310 | 87695..87952 | - | 258 | WP_082045657.1 | transposase | - |
Achr_RS22315 | 88277..88663 | + | 387 | Protein_95 | IS4 family transposase | - |
Achr_RS22320 | 88777..88959 | + | 183 | WP_040107493.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
Achr_RS22325 | 88991..89395 | + | 405 | WP_040107336.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
Achr_RS22330 | 89521..89784 | + | 264 | WP_040107494.1 | hypothetical protein | - |
Achr_RS22335 | 90048..91133 | + | 1086 | WP_039807303.1 | IS5 family transposase | - |
Achr_RS22340 | 91399..92007 | + | 609 | WP_040107337.1 | cupin | - |
Achr_RS22345 | 92097..93320 | - | 1224 | WP_052264083.1 | OprD family porin | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | - | - | 1..311724 | 311724 | |
- | inside | IScluster/Tn | - | - | 87496..91133 | 3637 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 61 a.a. Molecular weight: 6800.85 Da Isoelectric Point: 10.4803
>T52387 WP_040107493.1 NZ_CP010421:88777-88959 [Azotobacter chroococcum NCIMB 8003]
MRSREVIELLEADGWYEVAVKGSHHQFKHPTKPGRVTVPHPKSDLPKGTWQSILKQAGLK
MRSREVIELLEADGWYEVAVKGSHHQFKHPTKPGRVTVPHPKSDLPKGTWQSILKQAGLK
Download Length: 183 bp
>T52387 NZ_CP010421:88777-88959 [Azotobacter chroococcum NCIMB 8003]
ATGCGAAGTCGGGAAGTGATCGAGCTGCTGGAGGCGGATGGTTGGTACGAAGTGGCGGTGAAAGGTAGTCACCACCAGTT
CAAGCACCCGACCAAGCCGGGGAGGGTGACGGTTCCGCACCCGAAATCGGATCTGCCGAAAGGCACCTGGCAGAGCATCC
TGAAACAAGCCGGCCTCAAGTGA
ATGCGAAGTCGGGAAGTGATCGAGCTGCTGGAGGCGGATGGTTGGTACGAAGTGGCGGTGAAAGGTAGTCACCACCAGTT
CAAGCACCCGACCAAGCCGGGGAGGGTGACGGTTCCGCACCCGAAATCGGATCTGCCGAAAGGCACCTGGCAGAGCATCC
TGAAACAAGCCGGCCTCAAGTGA
Antitoxin
Download Length: 135 a.a. Molecular weight: 14695.64 Da Isoelectric Point: 4.6292
>AT52387 WP_040107336.1 NZ_CP010421:88991-89395 [Azotobacter chroococcum NCIMB 8003]
MKYPVVIHKEQSSDYGVTVPDVPGCFSAGETMTEALENVREALELHFEGLLADGEPLPQPQEIDVHAANPDYAGGVWALV
DFDVMPYLGKAVRFNATLPEHLLARIDAYVKAHPEQKSRSGFLAEAALRVLQHA
MKYPVVIHKEQSSDYGVTVPDVPGCFSAGETMTEALENVREALELHFEGLLADGEPLPQPQEIDVHAANPDYAGGVWALV
DFDVMPYLGKAVRFNATLPEHLLARIDAYVKAHPEQKSRSGFLAEAALRVLQHA
Download Length: 405 bp
>AT52387 NZ_CP010421:88991-89395 [Azotobacter chroococcum NCIMB 8003]
ATGAAATATCCCGTCGTCATCCACAAAGAGCAGAGCAGCGACTACGGCGTGACCGTGCCGGACGTGCCAGGCTGCTTCTC
GGCCGGCGAGACGATGACCGAAGCGCTGGAGAATGTCCGTGAGGCGCTGGAGTTGCATTTCGAGGGGCTGCTGGCCGATG
GCGAGCCGCTGCCGCAGCCGCAGGAGATCGATGTGCACGCGGCGAACCCGGACTATGCCGGCGGCGTGTGGGCGCTGGTT
GACTTCGATGTGATGCCCTATCTGGGGAAGGCAGTGCGCTTCAACGCCACGCTGCCGGAGCACCTGCTGGCGCGGATCGA
TGCCTACGTGAAGGCCCATCCGGAGCAGAAGAGCCGCTCTGGATTTCTGGCTGAGGCGGCGCTGCGGGTGTTGCAGCACG
CTTGA
ATGAAATATCCCGTCGTCATCCACAAAGAGCAGAGCAGCGACTACGGCGTGACCGTGCCGGACGTGCCAGGCTGCTTCTC
GGCCGGCGAGACGATGACCGAAGCGCTGGAGAATGTCCGTGAGGCGCTGGAGTTGCATTTCGAGGGGCTGCTGGCCGATG
GCGAGCCGCTGCCGCAGCCGCAGGAGATCGATGTGCACGCGGCGAACCCGGACTATGCCGGCGGCGTGTGGGCGCTGGTT
GACTTCGATGTGATGCCCTATCTGGGGAAGGCAGTGCGCTTCAACGCCACGCTGCCGGAGCACCTGCTGGCGCGGATCGA
TGCCTACGTGAAGGCCCATCCGGAGCAGAAGAGCCGCTCTGGATTTCTGGCTGAGGCGGCGCTGCGGGTGTTGCAGCACG
CTTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A1I4HUC9 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0C4WXU9 |