52386

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/Phd(antitoxin)
Location 62088..62686 Replicon plasmid pAcX50f
Accession NZ_CP010421
Organism Azotobacter chroococcum NCIMB 8003

Toxin (Protein)


Gene name PfiT Uniprot ID A0A0C4WXT2
Locus tag Achr_RS22180 Protein ID WP_040107313.1
Coordinates 62345..62686 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0C4WSR8
Locus tag Achr_RS22175 Protein ID WP_040107312.1
Coordinates 62088..62348 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Achr_RS22165 57183..59024 + 1842 WP_040107310.1 amidohydrolase -
Achr_RS22170 59514..61706 + 2193 WP_040107311.1 catalase/peroxidase HPI -
Achr_RS22175 62088..62348 + 261 WP_040107312.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
Achr_RS22180 62345..62686 + 342 WP_040107313.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Achr_RS22185 62773..63072 + 300 WP_040107314.1 hypothetical protein -
Achr_RS22190 63258..64013 - 756 WP_040107315.1 precorrin-4 C(11)-methyltransferase -
Achr_RS22195 64010..64423 - 414 WP_040107316.1 cobalamin biosynthesis protein -
Achr_RS22200 64443..65126 - 684 WP_040107317.1 CbtA family protein -
Achr_RS22205 65134..65316 - 183 WP_040107318.1 CbtB-domain containing protein -
Achr_RS22210 66010..67059 + 1050 WP_040107319.1 cobalamin biosynthesis protein CobW -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..311724 311724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.98 Da        Isoelectric Point: 5.5128

>T52386 WP_040107313.1 NZ_CP010421:62345-62686 [Azotobacter chroococcum NCIMB 8003]
MSFDVQFATTAETSLFSQIAHLEPYHSYDGAYEKLSTLVVEVIRRLADHPLGYPISAQVSELGIAHYRELNLNGYRVFYE
CNEEKRLVVVLLILGQRQSIEQQLIHYCLMFDR

Download         Length: 342 bp

>T52386 NZ_CP010421:62345-62686 [Azotobacter chroococcum NCIMB 8003]
ATGAGCTTCGACGTTCAGTTTGCTACTACTGCCGAAACCAGCCTGTTTTCCCAGATCGCCCACCTAGAGCCGTACCACAG
CTATGACGGGGCCTATGAAAAGCTCTCCACCTTGGTGGTAGAAGTTATACGCCGCTTGGCCGATCACCCGTTGGGCTACC
CCATCAGTGCACAAGTCAGCGAGCTTGGGATCGCCCATTACCGGGAGCTCAATCTCAACGGCTACCGCGTCTTCTATGAG
TGCAATGAAGAAAAGCGTCTCGTGGTGGTCCTGCTCATCCTGGGGCAGCGACAGAGCATCGAGCAGCAGCTGATCCACTA
CTGCCTGATGTTTGATCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9543.04 Da        Isoelectric Point: 9.0005

>AT52386 WP_040107312.1 NZ_CP010421:62088-62348 [Azotobacter chroococcum NCIMB 8003]
MKIETISFLKKHAASLDLSEPMVITQNGLPAYVIESYAERKQRDETIALVKLLALGSRDKEQGKLVSGDTLLDRLAQRCQ
QPKGTS

Download         Length: 261 bp

>AT52386 NZ_CP010421:62088-62348 [Azotobacter chroococcum NCIMB 8003]
ATGAAAATCGAGACGATCAGCTTCTTGAAGAAGCACGCCGCATCGTTGGATCTGAGCGAGCCCATGGTGATCACCCAGAA
TGGTCTGCCCGCCTATGTCATCGAGTCCTATGCCGAACGCAAGCAGCGTGATGAGACGATTGCGCTCGTCAAGCTCCTGG
CATTGGGTTCCCGCGACAAAGAGCAAGGCAAGCTGGTGTCGGGTGACACGCTCCTGGATCGGTTGGCACAACGATGCCAG
CAACCCAAAGGAACTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C4WXT2

Antitoxin

Source ID Structure
AlphaFold DB A0A0C4WSR8

References