52385

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/Phd(antitoxin)
Location 37099..37697 Replicon plasmid pAcX50f
Accession NZ_CP010421
Organism Azotobacter chroococcum NCIMB 8003

Toxin (Protein)


Gene name PfiT Uniprot ID A0A0C4WSW8
Locus tag Achr_RS22055 Protein ID WP_040107297.1
Coordinates 37356..37697 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0C4WLQ2
Locus tag Achr_RS22050 Protein ID WP_040107296.1
Coordinates 37099..37359 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Achr_RS22025 32764..33807 + 1044 WP_040107291.1 L-glyceraldehyde 3-phosphate reductase -
Achr_RS22030 33810..34739 + 930 WP_040107292.1 aliphatic sulfonate ABC transporter substrate-binding protein -
Achr_RS22035 34829..35677 + 849 WP_040107293.1 energy transducer TonB -
Achr_RS22040 35711..36421 + 711 WP_040107294.1 MotA/TolQ/ExbB proton channel family protein -
Achr_RS22045 36423..36824 + 402 WP_040107295.1 biopolymer transporter ExbD -
Achr_RS22050 37099..37359 + 261 WP_040107296.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
Achr_RS22055 37356..37697 + 342 WP_040107297.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Achr_RS25605 37694..38158 + 465 WP_144411653.1 hypothetical protein -
Achr_RS22065 38256..38587 + 332 Protein_44 XRE family transcriptional regulator -
Achr_RS22070 38876..39862 - 987 WP_040107299.1 polysaccharide deacetylase family protein -
Achr_RS22075 39875..40411 - 537 WP_040107300.1 ester cyclase -
Achr_RS22080 40521..41435 + 915 WP_040107301.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..311724 311724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-98)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12985.72 Da        Isoelectric Point: 5.1029

>T52385 WP_040107297.1 NZ_CP010421:37356-37697 [Azotobacter chroococcum NCIMB 8003]
MSFDVQFATTAETSLFSQIAHLEPYHGYDGAYDKLSTLVAEVIRRLADHPLGYPISGQASELGITHYRELNLDGYRVFYE
CDEEKRLVVVLLILGQRQSNEQQLIHYCLMFAR

Download         Length: 342 bp

>T52385 NZ_CP010421:37356-37697 [Azotobacter chroococcum NCIMB 8003]
ATGAGCTTCGACGTTCAGTTTGCTACTACTGCTGAAACCAGCCTGTTTTCCCAGATCGCCCACCTGGAGCCGTACCACGG
CTATGACGGGGCCTATGACAAGCTCTCCACCTTGGTGGCAGAAGTTATACGCCGCCTGGCCGATCACCCGTTGGGCTACC
CCATCAGCGGACAAGCCAGCGAGCTTGGGATCACCCATTACCGGGAGCTCAATCTCGACGGCTACCGCGTCTTCTATGAG
TGCGACGAGGAAAAGCGCCTCGTGGTGGTCCTGCTCATCCTGGGGCAGCGACAGAGCAACGAACAGCAGCTGATCCACTA
CTGCCTGATGTTCGCTCGTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9567.10 Da        Isoelectric Point: 9.0005

>AT52385 WP_040107296.1 NZ_CP010421:37099-37359 [Azotobacter chroococcum NCIMB 8003]
MKIETISFLKKHAASLDLTEPMVITQNGLPAYVIESYAERKQRDETIALVKLLALGSRDKEQGKLVSGDTLLDRLAQRCQ
QPKGTP

Download         Length: 261 bp

>AT52385 NZ_CP010421:37099-37359 [Azotobacter chroococcum NCIMB 8003]
ATGAAAATCGAGACGATCAGCTTCTTGAAGAAGCACGCCGCATCGTTGGATCTAACCGAGCCAATGGTGATCACCCAGAA
TGGTCTGCCCGCCTATGTCATCGAGTCCTATGCCGAACGCAAGCAGCGCGATGAGACGATTGCGCTCGTCAAGCTCCTGG
CATTGGGTTCCCGCGACAAAGAGCAAGGCAAGCTGGTGTCGGGCGACACGCTCCTGGATCGGTTGGCACAACGATGCCAG
CAACCCAAAGGAACCCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C4WSW8

Antitoxin

Source ID Structure
AlphaFold DB A0A0C4WLQ2

References