Detailed information of TA system TA052383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 38007..38535
• Replicon: plasmid pAcX50c
• Accession: NZ_CP010418
• Organism: Azotobacter chroococcum NCIMB 8003

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0C4WUF3
• Locus tag: Achr_RS20835
• Protein ID: WP_040107086.1
• Coordinates: 38007..38288 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0C4WS17
• Locus tag: Achr_RS20840
• Protein ID: WP_040107087.1
• Coordinates: 38275..38535 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Achr_RS20815	33260..35065	+	1806	WP_040107082.1	type I restriction-modification system subunit M	-
Achr_RS20820	35062..36195	+	1134	WP_040107083.1	DUF1016 family protein	-
Achr_RS20825	36192..37334	+	1143	WP_040107084.1	restriction endonuclease subunit S	-
Achr_RS20830	37479..37775	+	297	WP_040107085.1	HU family DNA-binding protein	-
Achr_RS20835	38007..38288	-	282	WP_040107086.1	type II toxin-antitoxin system YafQ family toxin	Toxin
Achr_RS20840	38275..38535	-	261	WP_040107087.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
Achr_RS20845	38593..39207	-	615	WP_040107088.1	recombinase family protein	-
Achr_RS25495	39590..42595	+	3006	WP_144411629.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..62783	62783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10819.28 Da
• Isoelectric Point: 6.7553

>T52383 WP_040107086.1 NZ_CP010418:c38288-38007 [Azotobacter chroococcum NCIMB 8003]
VRAIKQTSQFKRDLKREAKGQYQATLEDDLMPVLEALAQDHPLDIRHRDHALSGNWKDHRDCHIKPDLVLLYRKPDDETL
ELIRLGSHSELGL

Nucleotide

• Length: 282 bp

>T52383 NZ_CP010418:c38288-38007 [Azotobacter chroococcum NCIMB 8003]
GTGCGAGCGATTAAGCAAACCAGTCAATTCAAGCGCGACTTGAAACGCGAAGCGAAGGGGCAGTACCAGGCGACGCTCGA
GGATGACTTGATGCCCGTCCTCGAGGCCCTAGCGCAGGATCATCCGCTGGATATACGGCACCGTGATCATGCGCTGTCCG
GTAACTGGAAGGATCACCGTGACTGCCATATCAAGCCGGATCTGGTGCTTCTGTATCGGAAACCGGACGACGAAACGCTG
GAGCTGATTCGCCTCGGCTCGCATAGCGAGTTAGGCCTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9441.88 Da
• Isoelectric Point: 4.3895

>AT52383 WP_040107087.1 NZ_CP010418:c38535-38275 [Azotobacter chroococcum NCIMB 8003]
MATDTVVRARVDERVKEEATVVLKSMGLSMTDAIQMMLIRVAEEGRLPFEPLVPSLETIAAAREAREGNLETVTLDDLRA
AIRASD

Nucleotide

• Length: 261 bp

>AT52383 NZ_CP010418:c38535-38275 [Azotobacter chroococcum NCIMB 8003]
ATGGCTACCGATACGGTCGTGCGCGCCCGCGTGGACGAACGAGTGAAGGAGGAGGCTACCGTTGTGCTCAAATCAATGGG
GCTGTCGATGACGGATGCCATCCAGATGATGCTGATCCGCGTCGCCGAGGAGGGACGGCTCCCGTTCGAGCCGTTGGTGC
CATCCTTGGAAACCATCGCTGCTGCGCGGGAAGCGCGCGAGGGGAACCTCGAAACCGTGACCCTGGACGATCTACGGGCG
GCGATCCGTGCGAGCGATTAA

Structures

Toxin

• AlphaFold DB: A0A0C4WUF3

Antitoxin

• AlphaFold DB: A0A0C4WS17