Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 4195719..4197225 Replicon chromosome
Accession NZ_CP010415
Organism Azotobacter chroococcum NCIMB 8003

Toxin (Protein)


Gene name HipA1 Uniprot ID A0A0C4WKB2
Locus tag Achr_RS18995 Protein ID WP_039806632.1
Coordinates 4195954..4197225 (+) Length 424 a.a.

Antitoxin (Protein)


Gene name HipB1 Uniprot ID A0A0C4WW75
Locus tag Achr_RS18990 Protein ID WP_039806630.1
Coordinates 4195719..4195964 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Achr_RS18970 4190821..4191792 + 972 WP_039806625.1 glucokinase -
Achr_RS18975 4191873..4192454 - 582 WP_052263989.1 hypothetical protein -
Achr_RS18980 4193057..4194676 + 1620 WP_039806628.1 malate:quinone oxidoreductase -
Achr_RS18985 4194768..4195538 + 771 WP_082045517.1 haloacid dehalogenase type II -
Achr_RS18990 4195719..4195964 + 246 WP_039806630.1 helix-turn-helix transcriptional regulator Antitoxin
Achr_RS18995 4195954..4197225 + 1272 WP_039806632.1 type II toxin-antitoxin system HipA family toxin Toxin
Achr_RS19000 4197236..4197991 - 756 WP_039806634.1 metallophosphoesterase -
Achr_RS19005 4198063..4198881 - 819 WP_052263990.1 HNH endonuclease -
Achr_RS19010 4198890..4199747 - 858 WP_039806636.1 restriction endonuclease -
Achr_RS19015 4200009..4200236 - 228 WP_039806638.1 hypothetical protein -
Achr_RS19020 4200363..4201235 + 873 WP_039806641.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(11-66)


Sequences

Toxin        


Download         Length: 424 a.a.        Molecular weight: 47099.14 Da        Isoelectric Point: 8.0155

>T52382 WP_039806632.1 NZ_CP010415:4195954-4197225 [Azotobacter chroococcum NCIMB 8003]
MAHKLEVWLFAIRIGTLALVEGRLTFQYDSDWLKQPNAVALSCSLPLQAEPFDERRTRPFFAGLLPEGHLRRLIAQQLQV
SRQNDFALLDHIGGECAGAVTFLAPGQALPQPADSSEVEWLGNTQIITLLDELPRRPMLAGQDGLRLSLAGAQDKLPVVV
DGDRIGLPRNGTPSSHILKPAIHSVEDSITNEGFCLTLAEVMQLRPARSTIRSIRDRRFLLVERYDRTTDAEGRMQRLHQ
EDFCQALGVVPEMKYQNEGGPDLASCFALVRRVTRPSAPQVLRLLDYVVFNALIGNHDAHAKNFSLLYAGQAPVLAPLYD
MLSTAVYPNLTAKMAMKLGSQYRFGEVQARHWEQFAESAGLARAQARKRILELARLLPAAARKLQADPNHSFSSHALVER
IVNLIEQRCQLTIRRLTAPAAED

Download         Length: 1272 bp

>T52382 NZ_CP010415:4195954-4197225 [Azotobacter chroococcum NCIMB 8003]
ATGGCGCATAAGCTCGAGGTCTGGCTGTTTGCCATTCGCATTGGAACCTTGGCTCTGGTCGAGGGCCGCCTCACTTTCCA
GTACGACTCCGATTGGCTGAAGCAGCCGAATGCGGTCGCGCTGTCGTGCTCGCTACCGCTGCAGGCCGAGCCGTTCGACG
AGCGCAGGACACGACCGTTTTTCGCCGGCCTGCTGCCCGAAGGCCATCTGCGCCGCCTGATCGCACAGCAGCTCCAGGTA
TCCAGGCAGAACGACTTCGCCCTTCTGGACCACATCGGTGGCGAGTGTGCTGGAGCCGTCACCTTCCTGGCGCCGGGGCA
GGCCTTGCCCCAGCCTGCGGACAGCAGCGAGGTCGAGTGGCTGGGCAATACACAGATCATCACCCTTCTGGACGAGTTGC
CCCGCCGCCCCATGCTGGCAGGCCAGGATGGCCTGCGCCTTTCGCTAGCCGGCGCCCAGGACAAGCTTCCGGTGGTGGTC
GACGGGGATCGGATAGGACTGCCCCGCAACGGCACGCCAAGCTCGCACATCCTCAAGCCGGCCATTCACTCCGTCGAGGA
CAGCATAACCAACGAAGGATTCTGTCTGACACTGGCCGAAGTCATGCAACTGCGGCCCGCCCGCTCGACGATTCGCTCGA
TTCGCGATCGCCGCTTCCTTCTGGTCGAGCGCTATGACCGGACGACCGATGCCGAAGGGCGCATGCAGCGGCTGCATCAG
GAGGATTTCTGTCAGGCGCTGGGCGTGGTGCCCGAAATGAAATACCAGAACGAGGGCGGCCCCGATCTCGCAAGCTGCTT
TGCCTTGGTACGGCGTGTCACGCGCCCCAGCGCTCCCCAGGTGCTGCGCCTGCTGGATTACGTGGTCTTCAACGCCCTGA
TCGGCAACCACGACGCGCACGCCAAGAATTTCTCCCTGCTCTACGCCGGCCAGGCACCCGTGCTGGCTCCGCTCTATGAC
ATGCTATCGACGGCGGTATACCCGAACCTCACGGCCAAGATGGCGATGAAGCTCGGCAGCCAATACAGGTTCGGCGAAGT
GCAGGCCCGACACTGGGAGCAGTTTGCGGAAAGTGCCGGTCTTGCCAGAGCCCAAGCCCGCAAACGCATTCTCGAGCTAG
CCCGACTTCTGCCTGCGGCGGCCCGCAAACTCCAGGCCGATCCGAACCACAGCTTTTCCAGCCACGCTCTGGTCGAGCGG
ATCGTCAACCTGATCGAACAGCGCTGTCAGCTGACGATACGACGGCTGACAGCGCCGGCTGCCGAGGACTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8398.72 Da        Isoelectric Point: 10.7613

>AT52382 WP_039806630.1 NZ_CP010415:4195719-4195964 [Azotobacter chroococcum NCIMB 8003]
MTTIHTTQQLGKALRAARKQLGLTQSQLALAAGVGLRFIVDLEAGKPTLRLKNSLRVIEALGGEISLNGLPAPTGGDDHG
A

Download         Length: 246 bp

>AT52382 NZ_CP010415:4195719-4195964 [Azotobacter chroococcum NCIMB 8003]
ATGACCACTATTCATACCACCCAACAACTGGGCAAGGCTCTGCGCGCGGCCCGCAAGCAACTGGGGCTGACCCAGTCCCA
ACTGGCGCTGGCAGCCGGCGTCGGCTTGCGCTTCATCGTCGACCTGGAGGCCGGCAAGCCCACGCTGCGGCTGAAAAACA
GCCTGCGCGTGATCGAAGCACTGGGCGGCGAAATCAGCCTGAATGGCCTGCCAGCGCCTACCGGGGGTGACGATCATGGC
GCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C4WKB2

Antitoxin

Source ID Structure
AlphaFold DB A0A0C4WW75

References