Detailed information of TA system TA052381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3414098..3414735
• Replicon: chromosome
• Accession: NZ_CP010415
• Organism: Azotobacter chroococcum NCIMB 8003

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C4WPC1
• Locus tag: Achr_RS15310
• Protein ID: WP_039805675.1
• Coordinates: 3414098..3414505 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: A0A0C4WS69
• Locus tag: Achr_RS15315
• Protein ID: WP_039805676.1
• Coordinates: 3414505..3414735 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Achr_RS15280	3409805..3410587	+	783	WP_039805670.1	TatD family hydrolase	-
Achr_RS15285	3410726..3411568	-	843	WP_039805671.1	regulatory signaling modulator protein AmpE	-
Achr_RS15290	3411565..3412128	-	564	WP_039805672.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
Achr_RS15295	3412281..3413129	+	849	WP_039805673.1	carboxylating nicotinate-nucleotide diphosphorylase	-
Achr_RS15300	3413223..3413714	-	492	WP_052263967.1	GNAT family N-acetyltransferase	-
Achr_RS15305	3413711..3413971	-	261	WP_039805674.1	DUF1778 domain-containing protein	-
Achr_RS15310	3414098..3414505	-	408	WP_039805675.1	type II toxin-antitoxin system VapC family toxin	Toxin
Achr_RS15315	3414505..3414735	-	231	WP_039805676.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Achr_RS15320	3415004..3415249	+	246	WP_039805677.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
Achr_RS15325	3415246..3415632	+	387	WP_039805678.1	type II toxin-antitoxin system VapC family toxin	-
Achr_RS15330	3415738..3416280	-	543	WP_039805679.1	transposase	-
Achr_RS24295	3416541..3417053	-	513	WP_039805680.1	pilin	-
Achr_RS25245	3417231..3417473	+	243	WP_144411563.1	hypothetical protein	-
Achr_RS15340	3417599..3419299	+	1701	WP_039805681.1	type IV-A pilus assembly ATPase PilB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15118.26 Da
• Isoelectric Point: 8.0835

>T52381 WP_039805675.1 NZ_CP010415:c3414505-3414098 [Azotobacter chroococcum NCIMB 8003]
MSVRYLLDTNICIYIAKHNPPAVRERFARHSAAELAMSVITLGELRFGAEKSQARERALTVIRQLESLIQVAPLREEVGE
HYGQIRAGLQSQGLAIGNNDLWLAAHARSAGWTIVTNNVREFERVPGLQVENWVS

Nucleotide

• Length: 408 bp

>T52381 NZ_CP010415:c3414505-3414098 [Azotobacter chroococcum NCIMB 8003]
ATGTCGGTGCGTTACCTGCTGGATACCAACATCTGCATCTACATCGCCAAGCACAACCCGCCCGCCGTCAGGGAGCGCTT
TGCCCGTCACTCCGCCGCCGAACTGGCGATGTCCGTGATCACCTTGGGGGAGTTGCGTTTCGGCGCCGAGAAAAGCCAGG
CCAGGGAGCGGGCGCTGACCGTCATCCGGCAACTGGAGTCGCTGATCCAGGTCGCTCCCTTGCGGGAGGAGGTCGGCGAG
CACTACGGGCAGATCCGTGCCGGGCTGCAATCCCAGGGGTTGGCCATCGGCAACAACGACCTCTGGCTCGCTGCGCATGC
CCGCTCGGCGGGCTGGACCATCGTCACCAACAATGTGCGTGAATTCGAGCGTGTTCCGGGTTTGCAGGTGGAGAACTGGG
TGAGTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8550.78 Da
• Isoelectric Point: 4.7719

>AT52381 WP_039805676.1 NZ_CP010415:c3414735-3414505 [Azotobacter chroococcum NCIMB 8003]
MAFARVFKSGNSQAVRLPKEFRLDVERVEIFRRGDEIVLRVAPESGSVVFDALATLPADFMADGRQDMPPQEREAL

Nucleotide

• Length: 231 bp

>AT52381 NZ_CP010415:c3414735-3414505 [Azotobacter chroococcum NCIMB 8003]
ATGGCGTTCGCGCGGGTTTTCAAGTCCGGTAACAGTCAGGCGGTCCGCTTGCCCAAGGAGTTTCGTCTCGATGTCGAGCG
CGTCGAGATTTTTCGGCGTGGCGACGAGATCGTTCTGCGGGTGGCCCCGGAGAGCGGCAGTGTGGTTTTCGACGCGCTGG
CCACCCTTCCGGCCGATTTCATGGCGGATGGCCGCCAGGACATGCCCCCGCAAGAGCGCGAGGCGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A0C4WPC1

Antitoxin

• AlphaFold DB: A0A0C4WS69