Detailed information of TA system TA052378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2252068..2252632
• Replicon: chromosome
• Accession: NZ_CP010415
• Organism: Azotobacter chroococcum NCIMB 8003

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0C4WT50
• Locus tag: Achr_RS10170
• Protein ID: WP_039804037.1
• Coordinates: 2252345..2252632 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0C4WMB6
• Locus tag: Achr_RS10165
• Protein ID: WP_039804035.1
• Coordinates: 2252068..2252355 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Achr_RS10140	2247573..2247824	-	252	WP_039804027.1	hypothetical protein	-
Achr_RS25760	2247821..2247976	-	156	WP_158319651.1	hypothetical protein	-
Achr_RS10145	2247973..2248155	-	183	WP_039804029.1	hypothetical protein	-
Achr_RS10150	2248139..2248501	-	363	WP_039804031.1	hypothetical protein	-
Achr_RS10155	2251257..2251574	+	318	WP_039804033.1	DUF1654 domain-containing protein	-
Achr_RS10160	2251707..2251979	+	273	WP_039804034.1	hypothetical protein	-
Achr_RS10165	2252068..2252355	+	288	WP_039804035.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Achr_RS10170	2252345..2252632	+	288	WP_039804037.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Achr_RS10175	2252683..2253654	-	972	WP_039804039.1	hypothetical protein	-
Achr_RS10180	2253875..2254777	-	903	WP_082045412.1	endonuclease	-
Achr_RS10185	2254849..2255790	-	942	WP_039804041.1	helix-turn-helix domain-containing protein	-
Achr_RS10190	2255906..2256109	+	204	WP_039804044.1	helix-turn-helix domain-containing protein	-
Achr_RS10195	2256165..2256359	+	195	WP_039807734.1	hypothetical protein	-
Achr_RS10200	2256411..2256605	+	195	WP_039804045.1	hypothetical protein	-
Achr_RS10205	2256677..2257468	+	792	WP_082045414.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2242099..2286061	43962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-71)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10976.95 Da
• Isoelectric Point: 10.6762

>T52378 WP_039804037.1 NZ_CP010415:2252345-2252632 [Azotobacter chroococcum NCIMB 8003]
MAVEYAVEWAPQALKELRKLGGAIRLQLLRKLDERRRNPRVPGDALHGLKDCYKIKLKSAGYRLVYRVEDERVVVVVVAV
GKRERSAVYEQAMKR

Nucleotide

• Length: 288 bp

>T52378 NZ_CP010415:2252345-2252632 [Azotobacter chroococcum NCIMB 8003]
ATGGCCGTTGAATATGCGGTCGAGTGGGCGCCCCAGGCGCTGAAGGAGTTGCGCAAGCTCGGCGGGGCGATCCGTCTGCA
GCTGCTGCGAAAGCTCGACGAGCGCCGCCGTAACCCTCGCGTGCCGGGCGACGCACTGCACGGCCTCAAGGACTGCTACA
AGATCAAGCTGAAGTCCGCCGGATACCGGCTGGTCTACCGGGTTGAGGACGAGCGGGTGGTGGTAGTCGTGGTGGCCGTT
GGCAAACGGGAGCGTAGCGCCGTGTATGAGCAGGCGATGAAGCGGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10385.85 Da
• Isoelectric Point: 4.4280

>AT52378 WP_039804035.1 NZ_CP010415:2252068-2252355 [Azotobacter chroococcum NCIMB 8003]
MQSILADVAVSVSELKKNPSAVLSSAGGAPVAVLNHNRVMGYMVPADVFEAMMERLDDLELIRVARERIEANEPTVRVAL
EDLIAEAERDTEHGR

Nucleotide

• Length: 288 bp

>AT52378 NZ_CP010415:2252068-2252355 [Azotobacter chroococcum NCIMB 8003]
ATGCAGAGCATCTTGGCTGACGTCGCCGTTAGCGTTTCCGAGCTGAAGAAGAACCCTTCCGCCGTGCTGAGCAGCGCTGG
CGGCGCTCCGGTAGCCGTGCTGAACCACAATCGCGTCATGGGCTACATGGTGCCGGCTGACGTGTTCGAGGCCATGATGG
AGCGCCTGGACGATCTGGAGCTGATCCGGGTAGCTCGGGAGCGGATCGAGGCAAACGAGCCGACCGTCCGCGTGGCGCTG
GAAGACCTGATCGCCGAGGCCGAGCGCGATACTGAGCATGGCCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0C4WT50

Antitoxin

• AlphaFold DB: A0A0C4WMB6