52376

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 939473..940075 Replicon chromosome
Accession NZ_CP010415
Organism Azotobacter chroococcum NCIMB 8003

Toxin (Protein)


Gene name PumA Uniprot ID A0A0C4WQ16
Locus tag Achr_RS04250 Protein ID WP_039802152.1
Coordinates 939473..939763 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0C4WGT0
Locus tag Achr_RS04255 Protein ID WP_039802154.1
Coordinates 939773..940075 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Achr_RS04210 934694..934993 - 300 WP_052264003.1 hypothetical protein -
Achr_RS04215 935014..936849 - 1836 WP_082045363.1 DUF3987 domain-containing protein -
Achr_RS04220 936867..937757 - 891 WP_039802140.1 topoisomerase -
Achr_RS04225 937754..937960 - 207 WP_039802142.1 hypothetical protein -
Achr_RS04230 937957..938208 - 252 WP_039802144.1 hypothetical protein -
Achr_RS04235 938212..938517 - 306 WP_039802146.1 hypothetical protein -
Achr_RS04240 938514..938768 - 255 WP_039802148.1 hypothetical protein -
Achr_RS25915 938765..938956 - 192 WP_039802150.1 hypothetical protein -
Achr_RS25980 938953..939123 - 171 WP_167571670.1 hypothetical protein -
Achr_RS25725 939146..939541 - 396 Protein_862 ash family protein -
Achr_RS04250 939473..939763 + 291 WP_039802152.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Achr_RS04255 939773..940075 + 303 WP_039802154.1 putative addiction module antidote protein Antitoxin
Achr_RS04260 940086..940325 + 240 WP_082045364.1 BrnA antitoxin family protein -
Achr_RS04265 940558..940797 - 240 WP_039802156.1 hypothetical protein -
Achr_RS04270 940794..941465 - 672 WP_039802159.1 Rha family transcriptional regulator -
Achr_RS04275 941462..941770 - 309 WP_039802161.1 helix-turn-helix domain-containing protein -
Achr_RS04280 941770..941991 - 222 WP_039802163.1 transcriptional regulator -
Achr_RS04285 942512..943597 - 1086 WP_039807303.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 934064..969817 35753


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(5-96)

Antitoxin

Uncharacterized protein HI1420

(6-92)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 10676.31 Da        Isoelectric Point: 10.0976

>T52376 WP_039802152.1 NZ_CP010415:939473-939763 [Azotobacter chroococcum NCIMB 8003]
MPVLIQTDTYSKWFSALRDARAQARINARLRRVGLGTLGDCKPVGEGVSELRIDYGPGYRVYFVQRGHELVILLAGGDKS
SQDKDIKTAIKLARDL

Download         Length: 291 bp

>T52376 NZ_CP010415:939473-939763 [Azotobacter chroococcum NCIMB 8003]
ATGCCCGTACTCATTCAAACCGACACCTACAGCAAATGGTTCTCCGCCTTGCGGGACGCGCGAGCGCAAGCCCGTATCAA
CGCCAGGCTGCGCCGGGTAGGGCTTGGCACGTTGGGTGACTGCAAACCGGTGGGCGAAGGCGTGTCGGAATTGCGGATCG
ACTATGGCCCAGGCTACCGGGTGTATTTCGTCCAGCGTGGCCATGAGCTGGTCATCCTGCTGGCAGGCGGCGACAAGTCC
AGCCAGGACAAAGACATCAAAACAGCCATCAAGCTGGCGCGTGACCTGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 10676.35 Da        Isoelectric Point: 4.5836

>AT52376 WP_039802154.1 NZ_CP010415:939773-940075 [Azotobacter chroococcum NCIMB 8003]
MTKTTLTQWDVVDHLATEEDMALYLEACLEENDPALLAAALGDIARARGMAQLARDTGLTREGLYKALSVDGNPSLATIM
KVMGALGIKLHAELVPARPA

Download         Length: 303 bp

>AT52376 NZ_CP010415:939773-940075 [Azotobacter chroococcum NCIMB 8003]
ATGACGAAAACCACGCTGACCCAATGGGACGTCGTTGATCACCTGGCGACCGAAGAGGACATGGCCCTGTACTTGGAGGC
CTGCCTCGAAGAGAACGATCCCGCGCTGCTGGCTGCTGCGCTGGGCGATATTGCCCGTGCCCGCGGCATGGCCCAGCTCG
CCCGCGACACCGGCTTGACCCGCGAGGGGCTGTACAAGGCGCTGTCCGTAGACGGCAATCCCAGCCTGGCCACCATCATG
AAGGTGATGGGAGCACTGGGCATCAAGCTCCACGCCGAGCTTGTACCTGCCCGGCCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C4WQ16

Antitoxin

Source ID Structure
AlphaFold DB A0A0C4WGT0

References