Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1182830..1183385 Replicon chromosome
Accession NZ_CP010413
Organism Bifidobacterium breve strain BR3

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag RY69_RS05390 Protein ID WP_003829109.1
Coordinates 1183062..1183385 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag RY69_RS05385 Protein ID WP_003829112.1
Coordinates 1182830..1183075 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RY69_RS05365 1178682..1179344 + 663 WP_014483844.1 TetR/AcrR family transcriptional regulator -
RY69_RS05370 1179501..1180773 + 1273 Protein_1072 MFS transporter -
RY69_RS05375 1180957..1181135 - 179 Protein_1073 XRE family transcriptional regulator -
RY69_RS05380 1181400..1182656 - 1257 WP_015438708.1 HipA domain-containing protein -
RY69_RS05385 1182830..1183075 + 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
RY69_RS05390 1183062..1183385 + 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
RY69_RS10910 1183602..1184216 - 615 WP_003829107.1 cupin domain-containing protein -
RY69_RS05400 1184310..1185179 - 870 WP_003829104.1 class I SAM-dependent methyltransferase -
RY69_RS05405 1185417..1185782 + 366 WP_025299744.1 YccF domain-containing protein -
RY69_RS05410 1186324..1187589 + 1266 WP_060618603.1 Fic family protein -
RY69_RS10920 1188060..1188348 + 289 Protein_1081 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1158899..1193613 34714


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T52373 WP_003829109.1 NZ_CP010413:1183062-1183385 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T52373 NZ_CP010413:1183062-1183385 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT52373 WP_003829112.1 NZ_CP010413:1182830-1183075 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT52373 NZ_CP010413:1182830-1183075 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References