Detailed information of TA system TA052372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/DinJ(antitoxin)
• Location: 1122716..1123352
• Replicon: chromosome
• Accession: NZ_CP010413
• Organism: Bifidobacterium breve strain BR3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0M3T8E7
• Locus tag: RY69_RS05115
• Protein ID: WP_052787173.1
• Coordinates: 1122996..1123352 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: RY69_RS05110
• Protein ID: WP_016463214.1
• Coordinates: 1122716..1123009 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RY69_RS05085	1118200..1120125	+	1926	WP_016463219.1	alpha/beta hydrolase	-
RY69_RS05090	1120445..1121062	+	618	WP_141671914.1	hypothetical protein	-
RY69_RS05095	1121081..1121401	+	321	WP_016463217.1	hypothetical protein	-
RY69_RS05100	1121522..1122569	-	1048	Protein_1018	IS30 family transposase	-
RY69_RS05110	1122716..1123009	+	294	WP_016463214.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
RY69_RS05115	1122996..1123352	+	357	WP_052787173.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RY69_RS05120	1123529..1124131	-	603	WP_016463212.1	transglutaminase family protein	-
RY69_RS05125	1124236..1124592	-	357	WP_003833231.1	SdpI family protein	-
RY69_RS05130	1124682..1125167	+	486	WP_003829229.1	cytidine deaminase	-
RY69_RS05135	1125309..1126091	+	783	WP_016463211.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
RY69_RS05140	1126566..1127192	-	627	WP_003829226.1	30S ribosomal protein S4	-
RY69_RS05145	1127389..1128300	-	912	WP_016463209.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-53)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13313.23 Da
• Isoelectric Point: 7.9984

>T52372 WP_052787173.1 NZ_CP010413:1122996-1123352 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T52372 NZ_CP010413:1122996-1123352 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10866.37 Da
• Isoelectric Point: 4.2896

>AT52372 WP_016463214.1 NZ_CP010413:1122716-1123009 [Bifidobacterium breve]
MAIKEKATISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFMPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Nucleotide

• Length: 294 bp

>AT52372 NZ_CP010413:1122716-1123009 [Bifidobacterium breve]
ATGGCTATCAAAGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCATGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Toxin

• AlphaFold DB: A0A0M3T8E7