Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 2328155..2328710 Replicon chromosome
Accession NZ_CP010411
Organism Bifidobacterium longum subsp. infantis strain BT1

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag RY67_RS10030 Protein ID WP_003829109.1
Coordinates 2328387..2328710 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag RY67_RS10025 Protein ID WP_003829112.1
Coordinates 2328155..2328400 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RY67_RS12650 2323564..2323749 - 186 WP_164997102.1 hypothetical protein -
RY67_RS10010 2324213..2324875 + 663 WP_060621692.1 TetR/AcrR family transcriptional regulator -
RY67_RS10015 2325032..2326303 + 1272 WP_060621227.1 MFS transporter -
RY67_RS10020 2326725..2327981 - 1257 WP_003829116.1 HipA domain-containing protein -
RY67_RS10025 2328155..2328400 + 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
RY67_RS10030 2328387..2328710 + 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
RY67_RS11895 2328927..2329541 - 615 WP_082248120.1 cupin domain-containing protein -
RY67_RS10040 2329648..2330049 - 402 Protein_1984 class I SAM-dependent methyltransferase -
RY67_RS10045 2330287..2330652 + 366 WP_013140585.1 YccF domain-containing protein -
RY67_RS10050 2331195..2332460 + 1266 WP_060621229.1 Fic family protein -
RY67_RS10055 2332930..2333204 + 275 Protein_1987 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T52370 WP_003829109.1 NZ_CP010411:2328387-2328710 [Bifidobacterium longum subsp. infantis]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T52370 NZ_CP010411:2328387-2328710 [Bifidobacterium longum subsp. infantis]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT52370 WP_003829112.1 NZ_CP010411:2328155-2328400 [Bifidobacterium longum subsp. infantis]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT52370 NZ_CP010411:2328155-2328400 [Bifidobacterium longum subsp. infantis]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References