Detailed information of TA system TA052369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 2271181..2271817
• Replicon: chromosome
• Accession: NZ_CP010411
• Organism: Bifidobacterium longum subsp. infantis strain BT1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0M3T6K5
• Locus tag: RY67_RS09745
• Protein ID: WP_060621174.1
• Coordinates: 2271461..2271817 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: RY67_RS09740
• Protein ID: WP_060621171.1
• Coordinates: 2271181..2271474 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RY67_RS09715	2266566..2269244	-	2679	WP_038425923.1	alanine--tRNA ligase	-
RY67_RS12635	2269373..2269609	-	237	WP_007053386.1	hypothetical protein	-
RY67_RS09725	2269609..2270007	-	399	WP_007052133.1	DUF948 domain-containing protein	-
RY67_RS09730	2270090..2270764	-	675	WP_060621169.1	histidine phosphatase family protein	-
RY67_RS09735	2270864..2271109	+	246	Protein_1924	IS30 family transposase	-
RY67_RS09740	2271181..2271474	+	294	WP_060621171.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
RY67_RS09745	2271461..2271817	+	357	WP_060621174.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RY67_RS09750	2271994..2272596	-	603	WP_060621176.1	transglutaminase family protein	-
RY67_RS09755	2272701..2272982	-	282	Protein_1928	SdpI family protein	-
RY67_RS09760	2272969..2273340	+	372	Protein_1929	cytidine deaminase	-
RY67_RS09765	2273478..2274260	+	783	WP_060621178.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
RY67_RS09770	2274440..2275066	-	627	WP_007052130.1	30S ribosomal protein S4	-
RY67_RS09775	2275265..2276257	-	993	WP_082248114.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-109)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-53)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13272.18 Da
• Isoelectric Point: 8.9603

>T52369 WP_060621174.1 NZ_CP010411:2271461-2271817 [Bifidobacterium longum subsp. infantis]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLSLDDGPTGRLSAYDAGRVAWTINELYPGLLA

Nucleotide

• Length: 357 bp

>T52369 NZ_CP010411:2271461-2271817 [Bifidobacterium longum subsp. infantis]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTACGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTCCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCGCCTG
GACGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10914.39 Da
• Isoelectric Point: 4.3730

>AT52369 WP_060621171.1 NZ_CP010411:2271181-2271474 [Bifidobacterium longum subsp. infantis]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMYDPRIVTPQ
IRDGAVILPEGWDDDED

Nucleotide

• Length: 294 bp

>AT52369 NZ_CP010411:2271181-2271474 [Bifidobacterium longum subsp. infantis]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
ATTGAACCTGAGCACATTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGTATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Toxin

• AlphaFold DB: A0A0M3T6K5