Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1565451..1566088 Replicon chromosome
Accession NZ_CP010406
Organism Bacillus sp. Pc3

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag SB24_RS07280 Protein ID WP_003156187.1
Coordinates 1565451..1565801 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag SB24_RS07285 Protein ID WP_003156188.1
Coordinates 1565807..1566088 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SB24_RS07240 1560491..1561093 - 603 WP_039252641.1 SpoIIE family protein phosphatase -
SB24_RS07245 1561093..1561881 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
SB24_RS07250 1561847..1562329 - 483 WP_007609591.1 anti-sigma B factor RsbW -
SB24_RS07255 1562326..1562655 - 330 WP_031378636.1 anti-sigma factor antagonist -
SB24_RS07260 1562719..1563726 - 1008 WP_039252645.1 PP2C family protein-serine/threonine phosphatase -
SB24_RS07265 1563738..1564139 - 402 WP_003156178.1 anti-sigma regulatory factor -
SB24_RS07270 1564142..1564507 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
SB24_RS07275 1564512..1565333 - 822 WP_003156182.1 STAS domain-containing protein -
SB24_RS07280 1565451..1565801 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
SB24_RS07285 1565807..1566088 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
SB24_RS07290 1566208..1567377 - 1170 WP_039252648.1 alanine racemase -
SB24_RS07295 1567494..1568501 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
SB24_RS07300 1568666..1569031 - 366 WP_007410229.1 holo-ACP synthase -
SB24_RS07305 1569124..1569723 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T52362 WP_003156187.1 NZ_CP010406:c1565801-1565451 [Bacillus sp. Pc3]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T52362 NZ_CP010406:c1565801-1565451 [Bacillus sp. Pc3]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT52362 WP_003156188.1 NZ_CP010406:c1566088-1565807 [Bacillus sp. Pc3]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT52362 NZ_CP010406:c1566088-1565807 [Bacillus sp. Pc3]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References