Detailed information of TA system TA052353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2116843..2117372
• Replicon: chromosome
• Accession: NZ_CP010402
• Organism: Staphylococcus aureus strain GR2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RT44_RS10705
• Protein ID: WP_000621175.1
• Coordinates: 2116843..2117205 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RT44_RS10710
• Protein ID: WP_000948331.1
• Coordinates: 2117202..2117372 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RT44_RS10680	2113821..2114591	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
RT44_RS10685	2114566..2115045	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
RT44_RS10690	2115047..2115373	-	327	WP_001052491.1	anti-sigma factor antagonist	-
RT44_RS10695	2115492..2116493	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
RT44_RS10705	2116843..2117205	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RT44_RS10710	2117202..2117372	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RT44_RS10715	2117457..2118605	-	1149	WP_001281154.1	alanine racemase	-
RT44_RS10720	2118671..2119030	-	360	WP_000581197.1	holo-ACP synthase	-
RT44_RS10725	2119034..2119525	-	492	WP_001205912.1	PH domain-containing protein	-
RT44_RS10730	2119512..2121095	-	1584	WP_001294621.1	PH domain-containing protein	-
RT44_RS10735	2121088..2121567	-	480	WP_001287079.1	hypothetical protein	-
RT44_RS10740	2121776..2122336	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T52353 WP_000621175.1 NZ_CP010402:c2117205-2116843 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T52353 NZ_CP010402:c2117205-2116843 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT52353 WP_000948331.1 NZ_CP010402:c2117372-2117202 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT52353 NZ_CP010402:c2117372-2117202 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGTTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1